A seminested reverse transcription-PCR method coupled to membrane filtration was optimized to investigate the presence of norovirus (NV) RNA sequences in bottled and natural mineral waters. The recovery of viral particles by filtration varied between 28 and 45%, while the limit of detection of the overall method ranged from 6 to 95 viral particles. The assay was broadly reactive, as shown by the successful detection of 27 different viral strains representing 12 common genotypes of NVs. A total of 718 bottled and natural mineral water samples were investigated, including 640 samples of finished, spring, and line products (mostly 1 to 1.5 liters), collected from 36 different water brands of various types and from diverse geographic origins over a 2-year period. In addition, 78 samples of larger volume (10 and 400 to 500 liters) and environmental swabs were investigated. From the 1,436 analyses that were performed for the detection of NVs belonging to genogroups I and II, 34 samples (2.44%) were presumptively positive by seminested RT-PCR. However, confirmation by DNA sequence analysis revealed that all presumptive positive results were either due to nonspecific amplification or to cross-contamination. In conclusion, these results do not provide any evidence for the presence of NV genome sequences in bottled waters.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC262324 | PMC |
| http://dx.doi.org/10.1128/AEM.69.11.6541-6549.2003 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Field experiment confirms high macroplastic trapping efficiency of wood jams in a mountain river channel.
Sci Rep
January 2025
Institute of Nature Conservation, Polish Academy of Sciences, al. Adama Mickiewicza 33, 31- 120, Kraków, Poland.
Identifying macroplastic deposition hotspots in rivers is essential for planning cleanup efforts and assessing the risks to aquatic life and the aesthetic value of river landscapes. Recent fieldwork in mountain rivers has shown that wood jams retain significantly more macroplastic than other emergent surfaces within river channels. Here, we experimentally verify these findings by tracking the deposition of 64 PET bottles after 52-65 days of transport in the mid-mountain Skawa River (Polish Carpathians) under low to medium flow conditions.
View Article and Find Full Text PDFDietary and competition effects on life history attributes of Chrysomya megacephala and Lucilia sericata (Diptera: Calliphoridae) in south-west Europe.
Int J Legal Med
January 2025
University of Alicante, Department of Environmental Sciences and Natural Resources, PO Box 99, Alicante, E-03080, Spain.
The range of the oriental latrine fly (Chrysomya megacephala) is currently expanding. It coexists with another blowfly with a similar ecology, the green bottle fly (Lucilia sericata), one of the most abundant species in carrion during warm months. It is essential to understand the influence of temperature, larval substrate type, and larval competition on the development rates of these necrophagous calliphorids to evaluate the role and the adaptation of C.
View Article and Find Full Text PDFEvaluating the potential of Kalanchoe pinnata, Piper amalago amalago, and other botanicals as economical insecticidal synergists against Anopheles gambiae.
Malar J
January 2025
Caribbean Centre for Research in Biosciences, Natural Products Institute, University of the West Indies, Kingston, Jamaica.
Background: Synergists reduce insecticide metabolism in mosquitoes by competing with insecticides for the active sites of metabolic enzymes, such as cytochrome P450s (CYPs). This increases the availability of the insecticide at its specific target site. The combination of both insecticides and synergists increases the toxicity of the mixture.
View Article and Find Full Text PDFMultilevel analysis of prevalence and determinants of diarrhea among under-five children in Somalia: insights from the Somalia demographic and health survey 2020.
BMC Public Health
January 2025
Faculty of Science and Humanities, School of Postgraduate Studies and Research (SPGSR), Amoud University, Borama, Somaliland.
Background: Diarrheal diseases remain a critical public health challenge, particularly for children under five in low- and middle-income countries such as Somalia. This study aimed to assess the prevalence and determinants of diarrhea in this vulnerable population, utilizing data from the 2020 Somalia Demographic and Health Survey.
Methods: This investigation employed secondary data from the 2020 Somalia Demographic and Health Survey.
Stable H-O and radiogenic Sr isotopic ratios in bottled water in South Korea.
Isotopes Environ Health Stud
January 2025
Nakdong River Environment Research Center, National Institute of Environmental Research, Daegu, Republic of Korea.
Stable H-O and radiogenic Sr isotopic compositions of bottled water in South Korea were investigated to trace water origins and to elucidate hydrogeochemical water-rock interactions within aquifers. Eighty-one bottled water samples were collected across the country. The δD and δO values, and Sr/Sr ratios and Sr contents of groundwater-sourced bottled water samples were in the ranges of -68 to -40 ‰, -10 to -7 ‰, 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!