Four multiparous Holstein cows averaging 36 DIM and fitted with ruminal cannulas were utilized in a 4 x 4 Latin square design to investigate the effects of feeding extruded whole soybeans and Ca salts of long-chain fatty acids. Treatments were diets containing 1) no added fat, 2) 16% extruded whole soybeans, 3) 16% extruded whole soybeans and 3% Ca salts of long-chain fatty acids, and 4) 16% extruded whole soybeans and 6% Ca salts of long-chain fatty acids. Cows were fed for ad libitum intake a diet of alfalfa haylage, corn silage, and concentrate (35:15:50, DM basis). Intakes of DM and energy and production of milk, 4% FCM, fat, CP, and SNF were decreased by feeding extruded whole soybeans and 6% Ca salts of long-chain fatty acids, but differences were small among the other treatments. The weight percentages and yields of C18:0, C18:1, and C18:2 in milk were increased, and most shorter chain fatty acids were decreased, by feeding supplemental fat. Digestibilities of DM, OM, ADF, soluble residue, total C18 fatty acids, and total fatty acids were decreased, but digestibility of hemicellulose was increased by feeding supplemental fat. The largest decrease in digestibilities of most dietary constituents and in energy and N utilization occurred when 16% extruded whole soybeans plus 6% Ca salts of long-chain fatty acids were fed to the cows. Calcium salts of long-chain fatty acids can supply up to 3% of the dietary DM in diets containing 16% extruded whole soybeans without having deleterious effects on most variables measured in this experiment.
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http://dx.doi.org/10.3168/jds.S0022-0302(92)78064-3 | DOI Listing |
Adv Biotechnol (Singap)
March 2024
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Plant lipids are a diverse group of biomolecules that play essential roles in plant architecture, physiology, and signaling. To advance our understanding of plant biology and facilitate innovations in plant-based product development, we must have precise methods for the comprehensive analysis of plant lipids. Here, we present a comprehensive overview of current research investigating plant lipids, including their structures, metabolism, and functions.
View Article and Find Full Text PDFNeurosurg Rev
January 2025
Department of Critical Care Medicine, The Affiliated Taizhou People's Hospital of Nanjing Medical University, Zhou shan hui shui Community,199 Hailing South Road, Taizhou, Jiangsu Province, 225300, China.
Traumatic brain injury (TBI)-associated neuroinflammation and neurotoxicity can induce gastrointestinal dysfunction through the brain-gut axis. Partially hydrolyzed guar gum (PHGG) was demonstrated to exert beneficial health effects by altering gut microbiota and short-chain fatty acids (SCFAs) production. Our study aimed to explore the effects of PHGG on gastrointestinal dysfunction in TBI mouse models.
View Article and Find Full Text PDFPostgrad Med J
January 2025
Department of Medical Biochemistry, Faculty of Medicine, Niğde Omer Halisdemir University, Niğde, 51000, Turkey.
Background: Epoxyeicosatrienoic acids (EETs) are closely associated with lipoprotein metabolism, and changes in lipid profiles potentially affect their levels and functions. Given the alterations in lipid metabolism after cholecystectomy, this study aimed to investigate the levels of four EET regioisomers (free and esterified) and lipid profiles in patients with cholelithiasis after laparoscopic cholecystectomy (LC) and explore correlations between these parameters.
Methods: This prospective study involved 40 patients with symptomatic cholelithiasis who underwent LC.
Immunohorizons
January 2025
Agilex Biolabs, Adelaide, South Australia, Australia.
Enzyme-linked immunosorbent spot analysis is frequently used to investigate immune responsiveness during clinical trials. However, ELISpot classically utilizes peripheral blood mononuclear cell isolates from whole blood, requiring relatively high blood draw volumes and removing both granulocytes and bound drug. Here, we describe a novel protocol whereby CD45 cells are magnetically isolated from human whole blood and co-incubated with serum isolated from the same subject.
View Article and Find Full Text PDFAnal Methods
January 2025
Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Rapid and accurate methods for tracing and identifying the origin of lamb are crucial for ensuring food authenticity and quality. This study developed a precise traceability method to determine the origin of lamb by integrating rapid evaporative ionization mass spectrometry (REIMS) with multivariate statistical analysis. Lamb samples from Xilin Gol, Ordos, and Hulun Buir ranches were identified by REIMS fingerprinting within 1 min.
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