Rapid quantitative immuno-detection of haptens by the lateral flow assay without "typical" competitive inhibition results is studied. In the present study, we describe an immuno-threshold-based assay for the quantification of cortisol. It gives a signal which is directly proportional to the cortisol concentration in plasma samples with a performance time of only 5 min. This technique provides a practical calibration curve with detection limit of 3.5 ng/ml. The precision of the assay is 6% (intra-assay coefficient of variation, CV) and 10% (inter-assay CV). Cross-reactivity with related steroids is acceptably low: corticosterone (3.38%), cortisone (2.08%), deoxycorticosterone (2.00%), 17alpha-hydroxyprogesterone (0.39%), and progesterone (0.05%). Furthermore, the test strips show the advantages of long storage time and high stability that allow mass production and preparation of large batches. A one-step cortisol whole blood test derived from this plasma lateral flow assay has then been performed. It is a rapid chromatographic immunoassay designed for quantitative determination of cortisol in whole blood samples. It requires no sample pretreatment and gives result within 15 min. In principle, with this rapid and sensitive immunoassay, the immuno-test strips can be employed for detecting all low-molecular-weight haptens. It may also be a useful and convenient dipstick format for drug detection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jim.2003.07.009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Uncovering cerebral blood flow patterns corresponding to Amyloid-beta accumulations in patients across the Alzheimer's disease continuum using the arterial spin labeling.
Neurol Sci
January 2025
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Background: Alzheimer's disease (AD) is a progressive neurodegenerative disorder ranging from mild cognitive impairment (MCI) to AD dementia. Abnormal cerebral perfusion alterations, influenced by amyloid-beta (Aβ) accumulations, have been implicated in cognitive decline along this spectrum.
Objective: This study investigates the relationship between cerebrospinal fluid (CSF) Aβ1-42 levels and regional cerebral blood flow (CBF) changes across the AD continuum using the Arterial Spin Labeling (ASL) technique.
A hydrodynamic antenna: novel lateral line system in the tail of myliobatid stingrays.
Proc Biol Sci
January 2025
Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA.
Eagle rays, cownose rays and manta rays (order Myliobatiformes) have a slender tail that can be longer than the animal's body length, but its function and structure are unknown. Using histology, immunohistochemistry and three-dimensional imaging with micro-computed tomography scans, we describe the anatomy and function of the tail in , the cownose ray. The tail is an extension of the vertebral column with unique morphological specializations.
View Article and Find Full Text PDFEstablishment of DAS-ELISA and lateral flow immunochromatography for the detection of peanut allergen Ara h 3 after heat-moisture treatment.
Food Chem
January 2025
College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China. Electronic address:
Peanuts are highly nutritious but pose a significant risk of triggering food allergies. While heat treatment can reduce the allergenicity of many foods, it may also alter their structure, potentially impacting detection results. This study employed double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and lateral flow immunochromatography (LFIA) to evaluate the allergen Ara h 3 following heat-moisture treatment.
View Article and Find Full Text PDFSimultaneous or separate detection of heavy metal ions Hg and Ag based on lateral flow assays.
Mikrochim Acta
January 2025
Department of General Surgery, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, 266035, China.
A lateral flow assay (LFA) was developed for the simultaneous or separate detection of mercury ion and silver ion based on isothermal nucleic acid amplification. T-Hg-T and C-Ag-C were utilized in the isothermal nucleic acid amplification strategy to form specific complementary base pairs. Under the action of KF polymerase and endonuclease Nt.
View Article and Find Full Text PDFThree-dimensional aortic arch geometry and blood flow in neonates after surgical repair for aortic coarctation.
Front Cardiovasc Med
January 2025
Pediatric Cardiology, Pediatric Heart Center, Skåne University Hospital, Lund, Sweden.
Background: Recurrent coarctation of the aorta (re-CoA) is a well-known although not fully understood complication after surgical repair, typically occurring in 10%-20% of cases within months after discharge.
Objectives: To (1) characterize geometry of the aortic arch and blood flow from pre-discharge magnetic resonance imaging (MRI) in neonates after CoA repair; and (2) compare these measures between patients that developed re-CoA within 12 months after repair and patients who did not.
Methods: Neonates needing CoA repair, without associated major congenital heart defects, were included.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!