Yersinia enterocolitica synthesizes N-acyl-L-homoserine lactone (AHL) signal molecules via the LuxR-LuxI homologues YenR-YenI. In this study we checked two prototypes of mouse-virulent Y. enterocolitica serotype O8 strains WA-314 and 8081 for AHL production in vitro and in vivo (mouse infection model). We used thin-layer chromatography in combination with the Escherichia coli AHL biosensor to identify the AHL species produced. We detected only OHHL [N-(3-oxohexanoyl)-L-homoserine lactone] and not HHL (N-hexanoyl-L-homoserine lactone) produced by Y. enterocolitica O8 in culture supernatant or infected mouse tissue. This is the first report demonstrating AHL production by yersiniae during infection.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC219576 | PMC |
| http://dx.doi.org/10.1128/IAI.71.11.6624-6626.2003 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prevalence of common diarrheagenic enterobacteriaceae in Iran (2000-2023): a systematic review and meta-analysis.
BMC Gastroenterol
January 2025
Department of Biostatistics, Payame Noor University, Karaj, Iran.
Objective: Bacterial gastroenteritis is a significant public health concern, capable of causing severe infections. Among the various pathogens involved, those belonging to the Enterobacteriaceae family are the most frequently isolated and associated with gastrointestinal disorders. This study aimed to investigate the prevalence of common diarrheagenic Enterobacteriaceae in Iran over the past two decades, from 2000 to 2023.
View Article and Find Full Text PDFInvestigation into the effect of phenylalanine gating on anaerobic haem breakdown using the energy landscape approach.
Protein Sci
February 2025
Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.
We have recently demonstrated a novel anaerobic NADH-dependent haem breakdown reaction, which is carried out by a range of haemoproteins. The Yersinia enterocolitica protein, HemS, is the focus of further research presented in the current paper. Using conventional experimental methods, bioinformatics, and energy landscape theory (ELT), we provide new insight into the mechanism of the novel breakdown process.
View Article and Find Full Text PDFThyme Essential Oil as a Potential Tool Against Common and Re-Emerging Foodborne Pathogens: Biocidal Effect on Bacterial Membrane Permeability.
Microorganisms
December 2024
Department of Molecular Medicine and Medical Biotechnologies, University of Naples "Federico II", 80131 Naples, Italy.
Over the past decade, foodborne diseases have become a significant public health concern, affecting millions of people globally. Major pathogens like spp., , , and contaminate food and cause several infections.
View Article and Find Full Text PDFThymoquinone as a potent antimicrobial agent against Yersinia enterocolitica: Mechanisms of action and potential food safety applications.
Int J Food Microbiol
January 2025
College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China; Northwest A&F University ShenZhen Research Institute, Shenzhen, Guangdong 518057, China. Electronic address:
Yersinia enterocolitica (Y. enterocolitica) is a Gram-negative foodborne pathogen associated with potentially fatal diseases. Herein, the antibacterial activity and possible mechanism of thymoquinone (TQ) against Y.
View Article and Find Full Text PDFDetermination of bacteriocin genes and antimicrobial activity of Lactiplantibacillus plantarum isolated from feta cheese samples.
FEMS Microbiol Lett
January 2025
Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, 25240 Erzurum, Turkey.
In this study designed to isolate lactic acid bacteria (LAB) with bacteriocin production potential, white cheese samples were collected from different provinces of Turkey and isolation was carried out. A series of experiments were carried out for the main purpose and the actual bacteriocin producers were identified by detecting the genes encoding this bacteriocin. The experiments carried out in this direction were initially carried out with 20 isolates and as a result of various experiments, the number of isolates was reduced to 8 and the study was continued with 8 isolates.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!