AI Article Synopsis
- The study explored how human bone marrow stromal cells (BMSCs) interact with an RGD-modified silk matrix, showing improved adhesion, spreading, and proliferation compared to non-RGD-modified matrices.
- Results from scanning electron microscopy (SEM) and MTT analyses indicated that RGD-modified matrices supported a higher attachment and cell density of BMSCs and anterior cruciate ligament fibroblasts (ACLFs) over a 14-day culture period.
- The RGD-modified silk matrices resulted in significantly greater collagen type I levels at both day 7 and day 14, highlighting their potential application in tissue engineering due to their supportive properties for cell attachment and biocompatibility.
Article Abstract
Adhesion, spreading, proliferation, and collagen matrix production of human bone marrow stromal cells (BMSCs) on an RGD-modified silk matrix was studied. Anterior cruciate ligament fibroblasts (ACLFs) were used as a control cell source. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the modified silk matrices support improved BMSC and ACLF attachment and show higher cell density over 14 days in culture when compared with the non-RGD-modified matrices. Collagen type I transcript levels (at day 7) and content (at day 14) was significantly higher on the RGD-modified substrate than on the nonmodified group. The ability of RGD-coupled silk matrices to support BMSC attachment, which leads to higher cell density and collagen matrix production in vitro, combined with mechanical, fatigue, and biocompatibility properties of the silk protein matrix, suggest potential for use of this biomaterial for tissue engineering.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jbm.a.10120 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!