The presence of abundant cholesterol crystals symbolizes the disorder of cholesterol metabolism during the development of atherosclerosis. Examination of cultured human THP-1 macrophages treated with the cholesterol oxide, 7-ketocholesterol, revealed a concentration- and time-dependent increase in formation of cholesterol crystals in the cells. Radioisotope labeling and X-ray diffraction confirmed the presence of 7-ketocholesterol crystalline domains (d space 35.8A). Under the normal cell culture condition (5% CO(2), 37 degrees ), incubation with 7-ketocholesterol induced moderate levels of apoptosis. Elevating temperature from 37 to 40 degrees markedly reduces formation of the crystals in the macrophages. Meanwhile, at high temperatures, significantly increased numbers of apoptotic cells were detected in the cells treated with 7-ketocholesterol but not in those with native free cholesterol. These results suggest that hyperthermia inhibits cholesterol crystallization and promotes apoptotic effects of oxysterols on macrophages.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0006-2952(03)00502-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Varying pixel resolution significantly improves deep learning-based carotid plaque histology segmentation.
Sci Rep
January 2025
Medical Physics, University of Wisconsin School of Medicine and Public Health (UW-SMPH), Madison, USA.
Carotid plaques-the buildup of cholesterol, calcium, cellular debris, and fibrous tissues in carotid arteries-can rupture, release microemboli into the cerebral vasculature and cause strokes. The likelihood of a plaque rupturing is thought to be associated with its composition (i.e.
View Article and Find Full Text PDFAn autopsy case of cholesterol crystal embolization following alteplase administration for acute ischemic stroke.
Neurol Sci
January 2025
Department of Diagnostic Pathology, Asama Nanroku Komoro Medical Center, Komoro, Nagano, Japan.
Apolipoprotein B-containing lipoproteins in atherogenesis.
Nat Rev Cardiol
January 2025
Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
Article Synopsis
- ApoB is the key protein found in LDL and other lipoproteins, playing a major role in their formation and link to atherosclerosis.
- LDL contributes to plaque formation in arteries by entering the wall and triggering inflammatory responses through interaction with other molecules, leading to harmful processes like foam cell formation.
- Research has identified potential interventions to combat atherosclerosis by lowering lipoprotein levels and addressing the inflammatory responses in the arterial wall.
Insights into hepatotoxicity of fluorinated liquid crystal monomer 1-ethoxy-2,3-difluoro-4-(trans-4-propylcyclohexyl) benzene (EDPrB) in adult zebrafish at environmentally relevant concentrations: Metabolic disorder and stress response.
J Hazard Mater
December 2024
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Fluorinated liquid crystal monomers (FLCMs) are widely employed in liquid crystal display (LCD) panels. As emerging environmental contaminants with persistent, bioaccumulative, and toxic properties, FLCMs were proven to accumulate in liver, raising great concern regarding potential hepatotoxicity. 1-Ethoxy-2,3-difluoro-4-(trans-4-propylcyclohexyl) benzene (EDPrB), as one representative FLCM, was chosen to investigate the hepatotoxicity in adult zebrafish (Danio rerio) at environmentally relevant concentrations (1, 10, and 100 μg/L) with long-term exposure (21 days).
View Article and Find Full Text PDFRecovery and Stabilization of Kidney Allograft Function Following Post-Implantation Cholesterol Crystal Embolization.
Clin Transplant
December 2024
Department of Nephrology, Sundaram Hospital, Trichy, Tamil Nadu, India.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!