AI Article Synopsis
- This paper evaluates a plant-derived antibody specific to HBsAg for purifying the recombinant HBsAg used in vaccines.
- It details the efficient coupling of antibodies from 100-200kg tobacco leaves to Sepharose CL-4B, achieving comparable purification results to mouse-derived antibodies.
- The findings suggest that using plant-derived antibodies for HBsAg immunopurification is feasible, providing an alternative to the traditional use of monoclonal antibodies from mice.
Article Abstract
This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurification of the recombinant HBsAg for vaccine purposes. This plant-derived antibody was obtained from different batches of 100-200kg of tobacco leaves and coupled to Sepharose CL-4B with high efficiency. The plant-derived antibody immunoaffinity matrix purification behavior (elution capacity, antigen purity, purification cycles, and ligand leakage) was comparable to that of its mouse-derived monoclonal antibody homolog. This result supports the feasibility of using this plant-derived antibody for the immunopurification of the Hepatitis B surface antigen for human use, opening a new possibility to overcome the constrain of monoclonal antibody production in mice.
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| http://dx.doi.org/10.1016/j.bbrc.2003.08.149 | DOI Listing |
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