[reaction: see text] Biotin was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamides at the N-10 or 9-position carboxamide. Upon binding to avidin, the light output of the N-10 derivative (8) was quenched up to 92% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (9) was quenched only 33%. The utility of this effect was demonstrated in a model homogeneous chemiluminescence assay.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ol035323p | DOI Listing |
Publication Analysis
Top Keywords
9-position carboxamide
8
regiodependent luminescence
4
luminescence quenching
4
quenching biotinylated
4
biotinylated n-sulfonyl-acridinium-9-carboxamides
4
n-sulfonyl-acridinium-9-carboxamides avidin
4
avidin [reaction
4
[reaction text]
4
text] biotin
4
biotin conjugated
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!