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Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli.
Sci Rep
January 2025
Dabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal University, Xinyang, 464000, Henan, China.
Hydroxytyrosol, a fine chemical, is widely utilized in food and pharmaceutical industries. In this study, we constructed a pathway to produce hydroxytyrosol by co-expressing tyrosin-phenol lyase (TPL), L-amino acid dehydrogenase (aadL), α-keto acid decarboxylase (KAD), aldehyde reductase (yahK) and glucose dehydrogenase (gdh). We changed combinations between plasmids with different copy numbers and target genes, resulting in 84% increase in hydroxytyrosol production.
View Article and Find Full Text PDFA computational strategy to improve the activity of tyrosine phenol-lyase for the synthesis of L-DOPA.
Sci Rep
October 2024
Hangzhou Institute of Medicine, Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, 310000, China.
Enzymes with high catalytic activity and stability are essential for industrial production, yet most natural enzymes do not meet these requirements. Therefore, efficient strategies for enzyme engineering are crucial. In this study, we developed a cost-effective computational design strategy to enhance the activity of tyrosine phenol-lyase (TPL) for the production of L-DOPA.
View Article and Find Full Text PDFLignin-based monophenolic model compounds in L-tyrosine derivative synthesis via tyrosine phenol lyase.
Enzyme Microb Technol
December 2024
Chemical Process Engineering Research Unit, University of Oulu, P.O. Box 4300, Oulu 90014, Finland.
Tyrosine phenol lyase (TPL) synthesises L-tyrosine derivatives from monophenols, pyruvate and ammonia. Production of such high-value aromatic chemicals from biomass-derived raw materials is of great interest. In this study, six monophenols (guaiacol, phenol, o-cresol, m-cresol, catechol and syringol) were chosen based on the structure of lignin and were studied as substrates in the enzymatic reaction.
View Article and Find Full Text PDF[Recent advances in pyridoxal phosphate-dependent enzymes and their applications].
Sheng Wu Gong Cheng Xue Bao
September 2024
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Article Synopsis
- Pyridoxal phosphate (PLP) is the active form of vitamin B6 and acts as a crucial coenzyme for several enzymes involved in key chemical reactions like racemization and transamination.
- The article explores the structure and function of specific PLP-dependent enzymes, such as ω-transaminase and lysine decarboxylase, highlighting their roles in biosynthesis and applications.
- It also discusses advancements in enzyme modification for industrial use, the potential for future developments including regeneration systems, and the promising biocatalytic applications of PLP-dependent enzymes.
Tyrosine phenol-lyase inhibitor quercetin reduces fecal phenol levels in mice.
PNAS Nexus
July 2024
Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Shizuoka, 4228526, Japan.
Article Synopsis
- Tyrosine phenol-lyase (TPL) is an enzyme in gut bacteria that produces phenol from L-Tyr, contributing to harmful uremic toxins linked to kidney issues.
- This study tested how dietary polyphenols, especially quercetin, can inhibit TPL and lower phenol production.
- Quercetin was found to be the most effective inhibitor of TPL and phenol production, indicating its potential as a treatment strategy for managing uremia despite its low absorption in the gut.
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