A 6 x 6 drop plate method for simultaneous colony counting and MPN enumeration of Campylobacter jejuni, Listeria monocytogenes, and Escherichia coli.

J Microbiol Methods

Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA.

Published: November 2003

A protocol was developed using 96-well plates and multichannel pipettes for serial dilutions, followed by drop plating on agar in a 6 x 6 format. This protocol permits simultaneous plating of six dilutions which greatly decreases the number of plates utilized thereby saving incubator space for organisms such as Campylobacter which require unique environmental conditions.

Download full-text PDF

Source
http://dx.doi.org/10.1016/s0167-7012(03)00194-5DOI Listing

Publication Analysis

Top Keywords

drop plate
4
plate method
4
method simultaneous
4
simultaneous colony
4
colony counting
4
counting mpn
4
mpn enumeration
4
enumeration campylobacter
4
campylobacter jejuni
4
jejuni listeria
4

Similar Publications

Species of the complex cause postbloom fruit drop of citrus and anthracnose fruit rot of strawberries. produces acervuli in diseased citrus flowers and strawberry fruit, surviving asymptomatically on vegetative tissues. Previous studies have suggested that dispersal mechanisms other than windblown rain may be involved in dispersal of conidia of sensu lato.

View Article and Find Full Text PDF

Genes and proteins expression profile of 2D vs 3D cancer models: a comparative analysis for better tumor insights.

Cytotechnology

April 2025

University Centre for Research and Development, University Institute of Pharmaceutical Sciences, Chandigarh University, Gharuan, Mohali, 140413 India.

When juxtaposed with 2D cell culture models, multicellular tumor spheroids demonstrate a capacity to faithfully replicate certain features inherent to solid tumors. These include spatial architecture, physiological responses, the release of soluble mediators, patterns of gene expression, and mechanisms of drug resistance. The morphological and behavioural similarities between 3D-cultured cells and cells within tumor masses highlight the potential of these models in studying cancer biology and drug responses.

View Article and Find Full Text PDF

Influence of age and gender on gait kinematics of pelvis and hip in healthy adults aged 19-60 years.

Front Bioeng Biotechnol

January 2025

MGM Centre of Human Movement Science, MGM School of Physiotherapy, MGM Institute of Health Sciences, Navi Mumbai, India.

Purpose: Pelvic and hip motion are pivotal in maintaining postural control and energy efficient gait. An insight into influence of age and gender on the coupled motion of hip and pelvis in gait-cycle will guide clinical rehabilitation strategies and pertinent technology-design for specific age-groups. Therefore, present study evaluated pelvic and hip-joint gait kinematics in healthy females and males across adult-hood.

View Article and Find Full Text PDF

Antimicrobial Activity of UV-Activated and Cysteamine-Grafted Polymer Foils Against Bacteria and Algae.

Polymers (Basel)

January 2025

Centre for Nanomaterials and Biotechnology, Faculty of Science, University of Jan Evangelista Purkyně, Pasteurova 15, 400 96 Ústí nad Labem, Czech Republic.

Surface modification of various polymer foils was achieved by UV activation and chemical grafting with cysteamine to improve surface properties and antimicrobial efficacy. UVC activation at 254 nm led to changes in surface wettability and charge density, which allowed the introduction of amino and thiol functional groups by cysteamine grafting. X-ray photoelectron spectroscopy (XPS) confirmed increased nitrogen and sulfur content on the modified surfaces.

View Article and Find Full Text PDF

This paper experimentally investigates the impact response of composite laminates made with conventional and bio-based epoxy resin. Drop tower impact tests were conducted at varying energy levels, including repeated low-energy impacts, to evaluate perforation resistance. The laminates' residual strength and damage tolerance were assessed using the Damage Index (DI) and by analysing the resonance frequency variations through the Impulse Excitation Technique (IET).

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!