AI Article Synopsis
- The study aimed to identify genes in the fetal heart that are crucial for heart muscle development and cell growth.
- Researchers used a technique called suppression subtractive hybridization on mRNA from fetal and adult heart cells to find differentially expressed genes.
- The results identified 39 fetal-dominant genes with potential roles in heart development, alongside various genes with unknown functions and high similarity to mitochondrial transcripts.
Article Abstract
Objective: To identify genes expressed in the fetal heart that are potentially important for myocardial development and cardiomyocyte proliferation.
Methods: mRNAs from fetal (29 weeks) and adult cardiomyocytes were use for suppression subtractive hybridization (SSH). Both forward (fetal as tester) and reverse (adult as driver) subtractions were performed. Clones confirmed by dot-blot analysis to be differentially expressed were sequenced and analyzed.
Results: Differential expressions were detected for 39 out of 96 (41%) clones on forward subtraction and 24 out of 80 (30%) clones on reverse. For fetal dominating genes, 28 clones matched to 10 known genes (COL1A2, COL3A1, endomucin, HBG1, HBG2, PCBP2, LOC51144, TGFBI, vinculin and PND), 9 clones to 5 cDNAs of unknown functions (accession AK021715, AF085867, AB040948, AB051460 and AB051512) and 2 clones had homology to hEST sequences. For the reverse subtraction, all clones showed homology to mitochondrial transcripts.
Conclusions: We successfully applied SSH to detect those genes differentially expressed in fetal cardiac myocytes, some of which have not been shown relative to myocardial development.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!