AI Article Synopsis
- A ribosome-associated gene called Rnssb-1 was isolated from the fungus Rhizopus nigricans, showing strong similarity to yeast chaperones.
- The gene's missing 3' end was found using 3' RACE, and it is continuously expressed without heat shock induction.
- Analysis confirmed that Rnssb-1 has multiple introns and is not affected by heat shock, with additional gene copies identified in the fungal genome.
Article Abstract
A ribosome-associated Hsp70-homologous gene (Rnssb-1) was isolated from the genomic library of the filamentous zygomycete fungus Rhizopus nigricans. The nucleotide sequence of a genomic clone encoded the N-terminal part of a protein with high similarity to the yeast SSB ribosome-associated chaperones. The missing 3' end of the gene was obtained by 3' RACE. The Northern blot analysis showed that the Rnssb-1 gene is constitutively expressed and is not induced upon heat shock at 37 degrees C. The primary structure analyses revealed that the coding region of the Rnssb-1 gene is interrupted by at least four introns. Their splicing was not inhibited by exposure of the organism to heat shock as proven by RT-PCR. A Southern blot analysis of R. nigricans genomic DNA confirmed the presence of two additional gene copies of ribosome-associated Hsp70 genes in the fungal genome.
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| http://dx.doi.org/10.1016/j.bbaexp.2003.08.003 | DOI Listing |
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Molecular characterization of a ribosome-associated Hsp70-homologous gene from Rhizopus nigricans.
Biochim Biophys Acta
October 2003
Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, Ljubljana, SI-1000, Slovenia.
Article Synopsis
- A ribosome-associated gene called Rnssb-1 was isolated from the fungus Rhizopus nigricans, showing strong similarity to yeast chaperones.
- The gene's missing 3' end was found using 3' RACE, and it is continuously expressed without heat shock induction.
- Analysis confirmed that Rnssb-1 has multiple introns and is not affected by heat shock, with additional gene copies identified in the fungal genome.
The HSP70 gene family in Pneumocystis carinii: molecular and phylogenetic characterization of cytoplasmic members.
J Eukaryot Microbiol
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Department of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0678, USA.
Pneumocystis carinii, a major opportunistic lung pathogen of AIDS patients, is found in a number of mammals and is proposed to be a member of the fungi. In this work, several members of the highly conserved HSP70 multigene family were characterized from rat-derived P. carinii.
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