During its life cycle Trypanosoma rangeli crosses the hemolymph of its invertebrate host. In the present study, we demonstrate for the first time the uptake of lipophorin (Lp), the main lipid-transporting particle of insect hemolymph. We observed that living T. rangeli parasites uptake lipids from both 32P- and 3H-, or 125I-labeled Lp. However, the parasites do not uptake any other hemolymphatic protein such as 32P-labeled vitellogenin. The presence of a specific receptor to Lp in the parasite surface is suggested based on experiments using 125I-Lp. We also investigated the intracellular fate of lipids using Texas Red-labeled phosphatidylethanolamine-Lp. Parasites were observed under confocal microscope and displayed fluorescent-labeled lipids close to the flagellar pocket and in vesicles at the posterior region. In conclusion, this study raises a novel set of molecular events which takes place during vector-parasite interaction.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2003.09.038 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Common Bed Bugs: Non-Viable Hosts for Parasites.
Cells
December 2024
Department of Biological Sciences, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
The hemoflagellate parasite is transmitted by triatomine kissing bugs and may co-infect humans together with its Chagas disease-causing congener . Using real-time quantitative polymerase chain reaction (RT-qPCR) and antimicrobial assays, we studied () the temporal and spatial distribution of in common bed bugs, , following oral ingestion and hemocoelic injection of and () the immune responses of bed bugs induced by infections. Irrespective of infection mode, no live were present in the bed bugs' hemolymph, salivary glands, or feces.
View Article and Find Full Text PDFHigh infection prevalences by Trypanosoma minasense in non-human primates from the Southeast region of Brazil.
Sci Rep
December 2024
Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Article Synopsis
- Non-human primates (NHPs) in Brazil were studied to investigate the infection rates of Trypanosoma minasense, with a focus on how landscape composition and forest fragmentation affected these rates.
- Sapajus nigritus showed the highest positivity for T. minasense in blood samples (60.9%), while other species like Callithrix spp. and Alouatta guariba clamitans had lower rates, but molecular analysis indicated significant infections in all groups.
- The study found a correlation between T. minasense infections and environmental factors: higher forest fragmentation led to increased infection rates in NHPs, suggesting that habitat loss may drive disease prevalence.
Hidden life cycle stages of the trypanosomatid Blastocrithidia triatomae Cerisola et al. 1971 (Protista: Excavata: Kinetoplastea) unveiled by a new cultivation method.
Zootaxa
June 2024
Laboratório de Biologia de Insetos; Universidade Federal Fluminense; Niterói/RJ; Brazil.
Blastocrithidia triatomae is a monoxenic trypanosomatid parasite of triatomines, sharing the same insect vectors with Trypanosoma cruzi Chagas, 1909 and T. rangeli Tejera, 1920. It is known to cause a complex syndrome in insects which induces severe metabolic disorders and increasing in mortality rates.
View Article and Find Full Text PDFRevisiting the development of Trypanosoma rangeli in the vertebrate host.
Mem Inst Oswaldo Cruz
November 2024
Fundação Oswaldo Cruz-Fiocruz, Instituto René Rachou, Grupo de Comportamento de Vetores e Interação com Patógenos, Belo Horizonte, MG, Brasil.
Background: Trypanosoma rangeli is a haemoflagellate parasite that infects triatomine bugs and mammals in South and Central America. Trypanosoma cruzi, the etiological agent of Chagas disease, has a partially overlapping geographical distribution with T. rangeli, that leads to mixed human infections and cross-reactivity in immunodiagnosis.
View Article and Find Full Text PDFExploring Trypanosoma cruzi transmission dynamics in an acute Chagas disease outbreak using next-generation sequencing.
Parasit Vectors
September 2024
Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.
Background: Chagas disease (CD), caused by Trypanosoma cruzi, poses a major global public health challenge. Although vector-borne transmission is the primary mode of infection, oral transmission is increasingly concerning.
Methods: This study utilized long-amplicon-based sequencing (long-ABS), focusing on the 18S rRNA gene, to explore T.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!