Guinea pig to rat orthotopic liver transplantation is associated with serious technical problems contributing to impaired graft reperfusion and a high incidence of primary non function. In order to reduce the operation time and thereby organ damage during procurement a simplified technique for reconstruction of the infrahepatic vena cava was developed and is described in detail. Reduced operation time was associated with markedly improved lobular graft perfusion and significantly better graft survival. We suggest a modification of the donor operation for the guinea pig to rat xenograft liver model for the sake of reducing non immunological factors in this difficult setting.
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