AI Article Synopsis
- Human skin fibroblasts can secrete human hepatocyte growth factor (hHGF), which is enhanced by protein kinase C (PKC) activators like phorbol esters but inhibited by dexamethasone.
- Pretreatment with phorbol 12-myristate 13-acetate (PMA) reduces hHGF secretion but results in the production of a biologically active form of hHGF.
- The growth-promoting activity of hHGF from PMA-treated cells can be neutralized by an anti-hHGF antiserum, suggesting the significance of PKC activation in hHGF production.
Article Abstract
Human skin fibroblasts secreted a certain amount of human hepatocyte growth factor (hHGF), as determined by an enzyme-linked immunosorbent assay for hHGF. This hHGF secretion was remarkably stimulated by protein kinase C (PKC)-activating phorbol esters, which was inhibited by the simultaneous addition of dexamethasone. Pretreatment with phorbol 12-myristate 13-acetate (PMA) caused a down-regulation in hHGF secretion. hHGF secreted by the PMA-treated cells showed a potent hepatocyte growth-promoting activity which was neutralized by an anti-hHGF antiserum. These results indicate both that PMA-treated human skin fibroblasts produce biologically active hHGF and the possible involvement of PKC activation in this process.
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| http://dx.doi.org/10.1016/0014-5793(92)80220-b | DOI Listing |
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