AI Article Synopsis
- Researchers developed oral-vaccine microspheres using inactivated Actinobacillus pleuropneumoniae serotype 1 antigens combined with enteric-coated polymers through a co-spray drying method.
- In a study with mice and pigs, the oral vaccine (AQ6-AP microspheres) showed comparable antibody responses and protection against bacterial challenges compared to traditional subcutaneous vaccines.
- The oral vaccine demonstrated superior protection in pigs, showing better survival rates and less lung damage than the intramuscular aluminum vaccine.
Article Abstract
Oral-vaccine microspheres based on formalin-inactivated Actinobacillus pleuropneumoniae serotype 1 (AP-1) antigens and enteric-coated polymers were prepared using a co-spray drying process. We evaluated using this for a peroral vaccine. We measured specific-antibody titers and protection from challenge in mouse and pig models. In mice (24 per group), a subcutaneous aluminum-adjuvant vaccine or oral vaccination with three doses of AQ6-AP microspheres provided similar protection against intranasal challenge with 5 x 10(8) colony-formation units (cfu) of AP-1 bacterial culture broth. Two weeks after four oral vaccinations with 600 mg of AQ6-AP microsphere acetate solution (containing formalin-inactivated AP-1 antigens of 1.0 x 10(10) cfu bacterial broth), pigs (9 per group) were challenged intranasally with 1 ml of AP-1 bacterial culture broth (5 x 10(9) cfu). The clinical signs, percentage of pig survival ratio, lung lesion areas, and microscopic examinations indicated that the oral AQ6-AP vaccine provided more protection than vaccinating pigs intramuscularly with AP-1 aluminum vaccine.
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| http://dx.doi.org/10.1016/s0167-5877(02)00195-2 | DOI Listing |
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Oral immunization using formalin-inactivated Actinobacillus pleuropneumoniae antigens entrapped in microspheres with aqueous dispersion polymers prepared using a co-spray drying process.
Prev Vet Med
September 2003
Department of Pathobiology, Pig Research Institute Taiwan, P.O. Box 23, Chu-Nan, Miaoli, Taiwan, ROC.
Article Synopsis
- Researchers developed oral-vaccine microspheres using inactivated Actinobacillus pleuropneumoniae serotype 1 antigens combined with enteric-coated polymers through a co-spray drying method.
- In a study with mice and pigs, the oral vaccine (AQ6-AP microspheres) showed comparable antibody responses and protection against bacterial challenges compared to traditional subcutaneous vaccines.
- The oral vaccine demonstrated superior protection in pigs, showing better survival rates and less lung damage than the intramuscular aluminum vaccine.
Release characteristics of microspheres prepared by co-spray drying Actinobacillus pleuropneumoniae antigens and aqueous ethyl-cellulose dispersion.
J Microencapsul
September 2001
Department of Pathobiology, Pig Research Institute Taiwan, Chu-Nan, Miaoli, Republic of China.
Using formalin inactivated Actinobacillus pleuropneumoniae antigens and aqueous ethylcellulose dispersions, microspheres of oral vaccines were developed by a co-spray drying process. The present study attempted to determine whether the dosage formulations of microspheres could form enteric matrices. To assess the enteric characteristics, an in vitro dissolution test was performed with the AQ6-AP microspheres; 95% of the A.
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Institute of Microbiology and Genetics, Section for Microbiology and Biotechnology, Biocenter, University of Vienna, A-1030, Vienna, Austria.
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Vet Microbiol
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Department of Large Animal Clinical Sciences Michigan State University, East Lansing 48824, USA.
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