Objective: To compare motility on sperm obtained from the vas deferens and incubated in seminal plasma (PS), a ionic medium similar to seminal plasma (MA) and HTF-HEPES culture medium.
Material And Method: Aspirates from de proximal vas deferens were obtained in eight healthy men during vasectomy and separated in three aliquots (5-6 millions sperm/mL). Aliquots were incubated on PS, MA and HTF-HEPES. Quantitative and qualitative motility were assessed at one hour intervals.
Results: Sperm motility decreased 30% in the 1st hour but at the 3rd hour it increased 70% in aliquots incubated in PS and MA. Straight line velocity (VSL) also increased significatively at the 1st and 3rd hour only in aliquots incubated in PS and MA compared to aliquots incubated in HTF-HEPES (1st hour: p < 0.01; 3rd hour: p < 0.001). Curvilinear velocity (VCL) decreased at 3 hours in aliquots incubated in PS and MA compared to HTF-HEPES (P < 0.001). VCL was lower on aliquots incubated in PS compared to MA (p < 0.01).
Conclusions: Sperm activation occurred in PS and MA at 3 hours. Incubation in HTF-HEPES culture medium resulted in a progressive decrease of sperm motility. This suggests that ionic/osmolar composition of PS is important for sperm activation of sperm obtained from the vas deferens.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Stability of Diluted Desmopressin Acetate Intranasal Solution at 0.01 mg/mL.
Int J Pharm Compd
December 2024
Université de Montréal, Montreal, Quebec, Canada.
Objective: In pediatrics, weight-based doses can be very small, leading to measuring tiny volumes of the commercial desmopressin nasal solution at 0.1mg/mL, which reduces precision and increases the risk of error. Since stability of the desmopressin acetate solution diluted at 0.
View Article and Find Full Text PDFIn vitro proteolysis mirrors intact muscle maturation in beef carcasses.
Meat Sci
February 2025
School of Animal Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. Electronic address:
An in vitro assay was developed to study protease activity during the maturation of beef postmortem. Myofibrils were purified from the semitendinosus and used as a sentinel for assessing the activity of endogenous proteases in longissimus thoracis et lumborum (LTL) and the extensor carpi radialis (ER) over time postmortem in beef carcasses. Samples were collected from each muscle at 0, 1, 2, 7, and 14 d of aging and snap frozen.
View Article and Find Full Text PDFEvaluation of GeneXpert MTB/Rif Ultra assay performance on formalin-fixed paraffin-embedded tissues for detection.
J Med Microbiol
October 2024
Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
We evaluated the Xpert MTB/Rif Ultra assay performance for (MTB) detection in formalin-fixed paraffin-embedded tissue (FFPET) compared to mycobacterial culture or laboratory-developed MTB PCR test (LDT). FFPET samples with histological features suggestive of tuberculosis from 2018 to 2023 were selected. Five hundred microlitres of tissue lysis buffer was added to FFPET scrolls and incubated at 75 °C for 5 min.
View Article and Find Full Text PDFAn evaluation of intravenous lipid emulsion on three common canine toxicants.
Front Vet Sci
September 2024
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virgina Tech, Blacksburg, VA, United States.
Objective: To determine whether intravenous lipid emulsion (ILE) therapy significantly reduces the concentration of baclofen, ibuprofen, and/or bromethalin in canine whole blood over time.
Animals: Seven 500 mL bags of canine DEA 1.1 negative blood were divided into aliquots of 125 mL and randomly assigned to one of three treatment groups (baclofen, ibuprofen, bromethalin) or four control groups (a positive control for each treatment group and a negative control group).
Cyclodextrin-Cholesterol Alone or in Combination With Cyclodextrin-Vitamin E Improves Bull Sperm Cryopreservation in a Soybean Lecithin Extender.
Reprod Domest Anim
October 2024
Department of Veterinary Sciences, Laboratoire Environnement, Santé et Production Animale (LESPA), Institute of Veterinary Sciences and Agronomic Sciences, University of Batna 1, Batna, Algeria.
Combining cholesterol-loaded methyl-β-cyclodextrin (CD-CHL) with vitamin E-loaded methyl-β-cyclodextrin (CD-Vit E) to combat cold shock and oxidative stress during sperm cryopreservation in soybean lecithin extenders remains unexplored. Thus, the current study aimed to investigate the effect of treating bull sperm with CD-CHL and CD-Vit E prior to cryopreservation in a soybean lecithin extender. Sperm collected from eight fertile bulls were pooled and split into six aliquots.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!