The enzymatic reaction by aerosol-OT (AOT)reverse micelles containing lipase in supercritical ethane was examined and is the focus of this paper. The reverse micelles were formed under various conditions at which their hydrodynamic diameters were measured by using the dynamic light scattering spectrophotometer. The reverse micelles in supercritical ethane were formed in the range of Wo (water/surfactant) less than six. The hydrodynamic diameter of the reverse micelles ranged from 2 to 5 microm. The hydrolysis reaction of triolein by the lipase in reverse micelles was also examined. The observations indicate that lipase in AOT reverse micelles in supercritical ethane showed activity. The conversion of triolein increased with the increase in size of reverse micelles and Wo, and reached its maximum near the critical temperature. Moreover, although the conversion of triolein increased with pressure, it was independent of pressure near the critical temperature.
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http://dx.doi.org/10.1007/s00449-002-0295-8 | DOI Listing |
Vaccines (Basel)
December 2024
Shenzhen Neocurna Biotechnology Corporation, 12/F, Block B, Building 1, Yinxingzhijie Phase II, Longhua District, Shenzhen 518100, China.
The endosomal escape of lipid nanoparticles (LNPs) is crucial for efficient mRNA-based therapeutics. Here, we present a cationic polymeric micelle (cPM) as a safe and potent co-delivery system with enhanced endosomal escape capabilities. We synthesized a cationic and ampholytic di-block copolymer, poly (poly (ethylene glycol) methacrylate--hexyl methacrylate)--poly(butyl methacrylate--dimethylaminoethyl methacrylate--propyl acrylate) (p(PEGMA--HMA)--p(BMA--DMAEMA--PAA)), via reversible addition-fragmentation chain transfer polymerization.
View Article and Find Full Text PDFNanomaterials (Basel)
January 2025
Laboratoire de Chimie de Coordination, CNRS & Université de Toulouse (UPS, INP), 31077 Toulouse, France.
Spin crossover (SCO) iron (II) coordination compounds in the form of nanohybrid SCO@SiO particles were prepared using a reverse micelles technique based on the TritonX-100/cyclohexane/water ternary system. Tetraethyl orthosilicate (TEOS) acts as precursor of both the SiF counter-anion and SiO to obtain Fe(NHtrz)(BF)(SiF)@SiO nanoparticles with different sizes and morphologies while modifying the TEOS concentration and reaction time. The adjustable mixed-anion strategy leads to a range of quite scarce abrupt spin crossover behaviors with hysteresis just above room temperature (ca.
View Article and Find Full Text PDFCarbohydr Polym
March 2025
Key Lab of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, 101 Longmian Avenue, Nanjing 211166, China. Electronic address:
In an effort to mitigate or reverse the pathological progression of early-stage osteonecrosis of the femoral head (ONFH), this study employed a promising strategy that involves the sustained delivery of osteogenic factors to augment core decompression, facilitated by the use of composite hydrogels. Specifically, a hydrogel was synthesized by blending chitosan, Pluronic F-127, and tripolyphosphate, utilizing both ionic bonding and copolymer micelle cross-linking techniques. This hydrogel demonstrated exceptional biocompatibility, temperature responsiveness, pH-dependent biodegradation, and controlled release properties.
View Article and Find Full Text PDFFood Chem
January 2025
School of Food Science and Engineering, Hainan University, Haikou 570228, China.
Due to the fact that association colloids were formed in krill oil, the oxidation mechanism of krill oil was more complicated. In this study, water-soluble ferrous sulfate (Fe(SO)), oil-soluble ferrous fumarate (CHFeO) and insoluble ferric oxide (FeO) were added to krill oil and stored at 60 °C for accelerated oxidation. Peroxide value, thiobarbituric acid reactive substances and aldehyde content showed that Fe(SO) had a stronger pro-oxidative effect.
View Article and Find Full Text PDFHypothesis: The oil phase controls the persistence length and aqueous channel diameter of reverse wormlike micelles (RWLMs), specifically by tuning the cohesive energy density of alkanes.
Experiments: We explore the influence of alkanes with varying chain lengths on the rheological properties, structural parameters, and morphology of RWLMs. To establish a link between the solvent characteristics and the structure of RWLMs, we employ a diverse set of complementary techniques, including rheological analysis, small-angle X-ray scattering (SAXS), Fourier-transform infrared (FT-IR) spectroscopy, and cryogenic transmission electron microscopy (cryo-TEM).
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