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Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China.
Vet Sci
January 2025
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
In order to investigate the bacterial species present in the conjunctival sacs of dogs with bacterial conjunctivitis in Wuhan (Hongshan District, Wuchang District, Jiangxia District, and Huangpi District) and their resistance to aminoglycoside antibiotics, samples of conjunctival sac secretions were collected from 56 dogs with bacterial conjunctivitis in various regions of Wuhan. Drug susceptibility testing for aminoglycoside antibiotics was performed on the most commonly isolated gram-positive and gram-negative bacteria. The expression of two aminoglycoside modifying enzyme genes, and , and three 16S rRNA methyltransferase genes, , and , were analyzed by PCR.
View Article and Find Full Text PDFReply to "Concerns regarding the interpretation of Shank3 protein isoforms expressed in Shank3B mice: potential off-target effects by a neomycin resistance cassette".
Mol Psychiatry
January 2025
Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA.
Optimizing genome editing efficiency in via a CRISPR/Cas9n-mediated editing system.
Appl Environ Microbiol
January 2025
State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, College of Life Sciences, Hubei University, Wuhan, China.
is an important bioresource to produce various antibacterial natural products, however, the time-consuming and labor-intensive genome editing toolkits hindered the construction and application of engineered strains, and this study aimed to establish an efficient CRISPR/Cas9n genome editing system in . Initially, the CRISPR/Cas9-mediated editing tool was employed to replace those awkward genome editing tools that relied on homologous recombination, while the off-target Cas9 exhibited high toxicity to Sf01. Therefore, the nickase mutation D10A, high-fidelity mutations including N497A, R661A, Q695A, and Q926A, and thiostrepton-induced promotor P were incorporated into the Cas9 expression cassette, which reduced its toxicity.
View Article and Find Full Text PDFEffectiveness of a single-dose phage cocktail on the reduction of multidrug-resistant Escherichia coli in suckling piglets.
Vet Microbiol
January 2025
Faculty of Animal Science and Technology, Maejo University, Sansai, Chiang Mai 50290, Thailand. Electronic address:
Antibiotics are commonly used in pig farming to control infections caused by diarrhea-causing Escherichia coli (E. coli). However, improper or excessive use of antibiotics in pigs can enhance antibiotic resistance (ABR).
View Article and Find Full Text PDFHighly Sensitive Spectrofluorimetric and Colorimetric Quantitation of Neomycin Using Nanosilver-Fluorescein Hybrid: A Comprehensive Assessment of Greenness and Blueness.
Luminescence
January 2025
Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Two versatile yet simple methods, colorimetric and spectrofluorimetric, were utilized for the quantitation of nonchromophore neomycin using silver nanoparticles modified with fluorescein. Fluorescein was excited at 485 nm (emission at 515 nm); when it is deposited on the surface of silver nanoparticles, its fluorescence intensity at 515 nm is quenched. Neomycin restores the fluorescence level at 515 nm by displacing fluorescein from nanoparticle binding sites.
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