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Optical histopathology based on the nonlabeling analysis with multiphoton excitation imaging.
Pathol Int
January 2025
Department of Pathology, Graduate School of Medicine, Osaka University, Osaka, Japan.
Histopathological diagnosis is the definitive method for the evaluation of disease status; however, some problems need to be solved, such as invasiveness, time consumption, and difficulty in three-dimensional observation. To overcome these problems, a novel observation method, distinct from conventional histology, using tissue sections and glass slides is desirable. Fluorescence imaging of human tissues with multiphoton excitation imaging (MpEI), which was originally used for intravital imaging in biological research, is a promising method.
View Article and Find Full Text PDFReal-Time Imaging Assessment of Stress-Induced Vascular Inflammation Using Heartbeat-Synchronized Motion Compensation.
Arterioscler Thromb Vasc Biol
December 2024
Department of Mechanical Engineering, KAIST (Korea Advanced Institute of Science and Technology), Daejeon, Korea (M.A.J., U.K., H.Y.).
Background: Chronic mental stress accelerates atherosclerosis through complicated neuroimmune pathways, needing for advanced imaging techniques to delineate underlying cellular mechanisms. While histopathology, ex vivo imaging, and snapshots of in vivo images offer promising evidence, they lack the ability to capture real-time visualization of blood cell dynamics within pulsatile arteries in longitudinal studies.
Methods: An electrically tunable lens was implemented in intravital optical microscopy, synchronizing the focal plane with heartbeats to follow artery movements.
Intravital Position Study of the Clinical Anatomy of the Middle Lobe and Superior Poles of the Thyroid Gland.
Medicina (Kaunas)
September 2024
Department of Operative and Clinical Surgery with Topographical Anatomy, I. I. Mechnikov Northwestern State Medical University of the Ministry of Health of the Russian Federation, 191015 Saint Petersburg, Russia.
: This study analyzes the issues of position dislocation of the head of the superior poles and lateral points of the outer edge of the middle divisions of each of the thyroid lobes (TL) changes. The purpose was to provide an intravital position study of the clinical anatomy of the middle and superior poles of the thyroid gland (TG). : We used data on anthropometry obtained during the study and data from MRI of the neck organs and anatomical formations of 100 healthy individuals, comprising 55 (55%) female and 45 (45%) male participants.
View Article and Find Full Text PDFIntravital microscopic thermometry of rat mammary epithelium by fluorescent nanodiamond.
Nanoscale Horiz
October 2024
Institute for Quantum Life Science, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
Quantum sensing using the fluorescent nanodiamond (FND) nitrogen-vacancy center enables physical/chemical measurements of the microenvironment, although application of such measurements in living mammals poses significant challenges due to the unknown biodistribution and toxicity of FNDs, the limited penetration of visible light for quantum state manipulation/measurement, and interference from physiological motion. Here, we describe a microenvironmental thermometry technique using FNDs in rat mammary epithelium, an important model for mammary gland biology and breast cancer research. FNDs were injected directly into the mammary gland.
View Article and Find Full Text PDFDynamic Pathology of Enteric Neural Network using Curcumin-assisted Multiphoton Laser Imaging in Hirschsprung Disease.
Ann Surg
September 2024
Departments of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine; Tsu, Japan.
Objective: In living tissue, it has been difficult to make microscopic-level observations without damaging the tissue.
Summary Background Data: We have invented a novel intravital fluorescent observation method (IFOM) for real-time tissue observation, combining multi-photon laser scanning microscopy (MPLSM) with curcumin vital staining (CVS-IFOM). The aim of this study was to use CVS-IFOM to analyze the enteric nervous system (ENS) in mice and human patients with hypoganglionosis and Hirschsprung disease.
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