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Pollen and Stigma Morphology, Pollen Viability and Stigma Receptivity of Wittmackia Species (Bromeliaceae) by Light, Fluorescence and Scanning Electron Microscopy.
Microsc Res Tech
January 2025
Programa de Pós-graduação Em Recursos Genéticos Vegetais, Universidade Federal Do Recôncavo da Bahia (UFRB), Programa de Pós-graduação Em Recursos Genéticos Vegetais, Cruz das Almas, Bahia, Brazil.
The genus Wittmackia has 44 species distributed in two centers of diversity: the Brazilian clade and the Caribbean clade. The Brazilian clade includes 29 species, with geographic distribution concentrated in the Northeast of Brazil. This study reports the morphology, ultrastructure, pollen viability and stigma receptivity by different microscopy techniques of 23 species of the genus Wittmackia endemic to Brazil and occurring in Atlantic Forest areas.
View Article and Find Full Text PDFEfficacy of salicylic acid (SA) in modulating the dynamics of pesticide-thiamethoxam-induced stress responses in Brassica juncea L. insights from biochemical and molecular dissection.
Environ Pollut
December 2024
Department of Botany, GDC, Pulwama, 192301, Jammu and Kashmir, India; Research and Development Cell, Lovely Professional University Punjab-144411, India. Electronic address:
The present study uncovers the impacts of pesticide-thiamethoxam (TMX- 750 mg L) and salicylic acid (SA- 0.01, 0.1 and 1 mM) in Brassica juncea L.
View Article and Find Full Text PDFEvaluation of Hepatotoxic Potential of an Azo Dye, Eriochrome Black T on Freshwater Catfish, Clarias batrachus.
Bull Environ Contam Toxicol
December 2024
Faculty of Life Sciences, Department of Zoology, Aligarh Muslim University, Aligarh, 202002, India.
Nowadays, textile industries are one of the major contributors to water pollution, causing a devastating impact on aquatic ecosystems. Therefore, the current study aimed to investigate the impact of a textile azo dye, Eriochrome Black T (EBT), on the liver of a freshwater fish, Clarias batrachus. Fish were exposed to three concentrations of EBT, i.
View Article and Find Full Text PDFProtocol for muscle fiber type and cross-sectional area analysis in cryosections of whole lower mouse hindlimbs.
STAR Protoc
December 2024
Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Capital Region, Denmark. Electronic address:
We outline a protocol to visualize all mouse lower hindlimb skeletal muscles simultaneously. We describe procedures for orientating the whole lower hindlimb in gum tragacanth prior to freezing, simplifying the proceeding experimental steps, and enhancing the comprehensiveness of characterizations. We then detail steps for quantifying muscle fiber size and fiber type characteristics in a single cryosection using histochemistry and immunofluorescence.
View Article and Find Full Text PDFHistochemical and molecular analyses reveal an insight into the scent volatiles synthesis and emission in ephemeral flowers of Murraya paniculata (L.) Jack.
Planta
October 2024
Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India.
Temporal histolocalization of floral volatiles in the petal epidermis of Murraya paniculata was found to be linked with the coordinated expression of candidate genes and successive accumulation of an internal pool of volatiles. Murraya paniculata (Rutaceae) is known for its highly fragrant ephemeral flowers that emit volatiles to attract nocturnal pollinators. To unfold the patterns of volatile emission in relation to floral life-span, we studied time-course accumulation and emission rate of scent volatiles at six timepoints of floral maturation, at an interval of 4 h starting from the bud stage to the senescence stage on the next day.
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