Serum iron determination.

J Clin Pathol

Published: May 1960

AI Article Synopsis

  • A new method for quickly and accurately measuring iron levels in serum or plasma is introduced, which works at room temperature.
  • The procedure requires either 2 ml of sample or 1 ml if iron levels are expected to be high, and it handles cloudy or jaundiced samples effectively.
  • An ethanolic solution is used to create a colored complex for iron, which can be measured using various photometers with specific cuvette sizes.

Article Abstract

A simple, rapid, and sensitive method for the determination of iron in serum or plasma is described. The procedure is carried out at room temperature with 2 ml. of serum or plasma, or with 1 ml. if high values are expected; it can be applied to turbid or jaundiced samples, whether previously frozen or not. An ethanolic solution of 4: 7-diphenyl-1: 10-phenanthroline is used to produce a coloured iron complex, the optical density of which can be measured in any suitable photometer, using either 10 or 20 mm. fused glass cuvettes or matched tubes of 1.1 cm. internal diameter.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC480059PMC
http://dx.doi.org/10.1136/jcp.13.3.241DOI Listing

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