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The dark side of fluorescent protein tagging - the impact of protein tags on biomolecular condensation.
Mol Biol Cell
January 2025
Department of Biology, Institute of Biochemistry, ETH (Eidgenössische Technische Hochschule) Zürich, 8093 Zürich, Switzerland.
Biomolecular condensation has emerged as an important mechanism to control various cellular processes through the formation of membraneless organelles. Fluorescent protein tags have been extensively used to study the formation and the properties of condensates and , but there is evidence that tags may perturb the condensation properties of proteins. In this study, we carefully assess the effects of protein tags on the yeast DEAD-box ATPase Dhh1, a central regulator of processing bodies (P-bodies), which are biomolecular condensates involved in mRNA metabolism.
View Article and Find Full Text PDFAssessment of nurses' knowledge and practice of pressure injuries prevention for critically ill patients in Rwanda: a cross-sectional study.
BMC Nurs
January 2025
College of Medicine and Health Sciences, School of Nursing and Midwifery, University of Rwanda, Po. Box: 3286, Kigali, Rwanda.
Background: Pressure injuries are costly and can lead to mortality and psychosocial consequences if not managed effectively. Proper management of pressure injuries is crucial for quality nursing care. However, there is limited research on nurses' knowledge and practices in preventing and managing pressure injuries among critically ill patients in Rwanda.
View Article and Find Full Text PDFMeasuring parents' awareness, identification, and communication practices about acquaintance abuse against children in Pakistan.
Arch Pediatr
January 2025
Department of Mass Communication, Allama Iqbal Open University, Islamabad, Pakistan.
Background: Child Sexual Abuse (CSA) is a growing healthcare issue worldwide. However, the acquittance rape is one of the underrepresented phenomena due to certain social and cultural concerns. Consequently, the role of parents is of greater significance to ensure the safety of their children outside and insider of their homes.
View Article and Find Full Text PDFThe WHO Disease Outbreak News during the Covid-19 pandemic.
PLOS Glob Public Health
January 2025
Center for Global Health Science and Security, Georgetown University Medical Center, Washington, DC, United States of America.
During the Covid-19 pandemic, the World Health Organization (WHO) was an important public source of information - not only about the pandemic, but also thousands of other potential health emergencies. Here, we examine the 242 reports published in the WHO Disease Outbreak News (DON) during the first four years of the Covid-19 pandemic (2020 to 2023), and document the diseases and regions that were reported. We find that multinational epidemics of diseases like Ebola virus and MERS-CoV continue to dominate the DON.
View Article and Find Full Text PDFMeasurement properties of the Trunk Impairment Scale-modified Norwegian version among people with mild to moderate multiple sclerosis.
Disabil Rehabil
January 2025
Faculty of Nursing and Health Sciences, Nord University, Bodø, Norway.
Purpose: The Trunk Impairment Scale-modified Norwegian version (TIS-modNV) measures trunk control for clinical and research purposes. This study examined the validity and reliability of the TIS-modNV in people with multiple sclerosis (pwMS).
Materials And Methods: Sixty-eight pwMS (mild to moderate) participated.
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