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Phanerochaete chrysosporium hyphae bio-crack, endocytose and metabolize plastic films.
J Hazard Mater
January 2025
School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China; Anhui Ecological Civilization Research Institute, Hefei University of Technology, Hefei 230009, China.
Numerous studies have focused on the effect and mechanism of plastic degradation; due to their high persistence, petroleum-based plastics are difficult for microbes to mineralize. Although such plastics have been demonstrated to be mineralized by white rot fungus, the reactions at the molecular level remain unknown. Here, we show the whole mineralization model of polyethylene film, that can be summarized as follows: 1) white rot fungus colonizes on polyethylene film, using additives as dissimilated carbon sources; 2) the fungus secretes extracellular enzymes protein, combining with stearic acid as electron donor, causes oxidation and cracking of polyethylene film; and 3) partial dissociated sub-microplastic debris access to cells, further oxidizes in sequential actions of intracellular enzymes, and ultimately mineralize via β-oxidation.
View Article and Find Full Text PDFApplication of aqueous two-phase extraction for separation and purification of various adeno-associated viruses.
Biotechnol Lett
January 2025
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang Key Laboratory of Smart Biomaterials, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.
Objective: Adeno-associated viruses (AAVs) are widely used as gene therapy vectors due to their safety, stability, and long-term expression characteristics. The objective of this work is to develop an aqueous two-phase system (ATPS) as a universal platform for the separation and purification of AAVs.
Results: This study utilized polyethylene glycol (PEG)/salt ATPSs to separate and purify various AAV serotypes, including AAV5, AAV8, and AAV9, which focusing on serotype-specific performance and partial empty capsid removal.
The effect of different beverages on the surface characteristics of PEEK and PEKK polymers.
Am J Dent
December 2024
Department of Biostatistics, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
Purpose: To evaluate the effects of various beverages on surface roughness and microhardness of PEEK and PEKK polymers.
Methods: Rectangular-shaped PEEK and PEKK polymers were fabricated and examined in the study. The specimens were immersed for 28 days at 37°C in red wine, coffee, and distilled water.
Rapid detection of microplastics in chicken feed based on near infrared spectroscopy and machine learning algorithm.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
College of Artificial Intelligence, Nankai University, Tianjin 300350, China.
The main objective of this study was to evaluate the potential of near infrared (NIR) spectroscopy and machine learning in detecting microplastics (MPs) in chicken feed. The application of machine learning techniques in building optimal classification models for MPs-contaminated chicken feeds was explored. 80 chicken feed samples with non-contaminated and 240 MPs-contaminated chicken feed samples including polypropylene (PP), polyvinyl chloride (PVC), and polyethylene terephthalate (PET) were prepared, and the NIR diffuse reflectance spectra of all the samples were collected.
View Article and Find Full Text PDFRobotic-Assisted Conversion of Unicompartmental Knee Arthroplasty to Total Knee Arthroplasty.
JBJS Essent Surg Tech
December 2024
Department of Orthopaedic Surgery, Cleveland Clinic Foundation, Cleveland, Ohio.
Background: Unicompartmental knee arthroplasty (UKA) procedures have become much more common in the United States in recent years, with >40,000 UKAs performed annually. However, it is estimated that 10% to 40% of UKAs fail and thus require conversion to total knee arthroplasty (TKA). In the field of total joint arthroplasty, robotic-assisted surgeries have demonstrated advantages such as better accuracy and precision of implant positioning and improved restoration of a neutral mechanical axis.
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