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Ni-induced selective precipitation of His-tagged recombinant proteins shortens purification time while maintaining high yield.
J Biotechnol
January 2025
Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei-shi, Tokyo 184-8588, Japan; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-8-1 Harumi-cho, Fuchu-shi, Tokyo 183-8538, Japan. Electronic address:
Nickel-NTA affinity chromatography is the current standard method for purifying His-tagged recombinant proteins. However, this process involves repetitive tasks, can be time-consuming, and reduces protein yield. Here, we present a simple, fast, and handy method for purifying His-tagged proteins using free Ni²⁺.
View Article and Find Full Text PDFTransforming healthcare performance monitoring - A cutting-edge approach with generalized additive profiles: GAMs for healthcare quality monitoring.
Medicine (Baltimore)
September 2024
Department of Mechanical Engineering, Rwanda Polytechnic/Integrated Polytechnic Regional College (IPRC) Karongi, Kigali, Rwanda.
Article Synopsis
- Recent research highlights an increasing use of statistical process control in healthcare data analysis, particularly in cardiology, necessitating the development of new methodologies due to diverse variables.
- The study utilized generalized additive models (GAMs) and two statistical methods—deviation (D) and Hotelling (T2)—to create control charts for monitoring strokes in patients, revealing that the T2 chart generally outperforms D in detecting medium-to-large process shifts.
- The findings suggest that these advanced statistical tools can enhance healthcare performance monitoring by effectively analyzing complex relationships among various health factors.
Species-level understanding of the bacterial community in Daqu based on full-length 16S rRNA gene sequences.
Food Microbiol
October 2024
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China; College of Life Science, University of Chinese Academy of Sciences, Beijing, 100049, PR China. Electronic address:
Daqu is used as the fermentation starter of Baijiu and contributes diversified functional microbes for saccharifying grains and converting sugars into ethanol and aroma components in Baijiu products. Daqu is mainly classified into three types, namely low (LTD), medium (MTD) and high (HTD) temperature Daqu, according to the highest temperatures reached in their fermentation processes. In this study, we used the PacBio small-molecule real-time (SMRT) sequencing technology to determine the full-length 16 S rRNA gene sequences from the metagenomes of 296 samples of different types of Daqu collected from ten provinces in China, and revealed the bacterial diversity at the species level in the Daqu samples.
View Article and Find Full Text PDFA Novel Method to Localize Patency Capsule by Ileocolonoscopy Facilitates Endoscopic Assessment of the Small and Large Intestine in Patients with Crohn's Disease.
Digestion
October 2024
Center for Inflammatory Bowel Disease, Tokyo Yamate Medical Center, Japan Community Healthcare Organization, Tokyo, Japan.
Introduction: Patients with Crohn's disease (CD) require an assessment of small bowel lesions, while difficulties exist in performing small intestinal examinations, especially in small-sized medical offices. Small bowel capsule endoscopy (SBCE) is handy and can be performed in most clinical settings. The only drawback of SBCE is a requirement of patency testing prior to the exam because it sometimes requires CT scanning to localize the ingested patency capsule (PC), which may be a substantial burden for the patient.
View Article and Find Full Text PDFMolecular Hydrogen Acts as a Hydrogen Bond Proton Acceptor: From Protonated Betaine Tagging to the Weakest Hydrogen Bond.
J Phys Chem A
May 2024
Institute of Chemistry, Faculty of Natural Sciences and Mathematics, SS Cyril and Methodius University, PO Box 162, 1000 Skopje, Republic of North Macedonia.
In an attempt to gain further insights into the intermolecular interactions implied by Rizzo's group's cautionary tale related to molecular tagging in infrared multiple photon dissociation (IRMPD) spectroscopy with molecular messengers [Masson, A. . 2015, 143, 104313], in the present study, we provide an in-depth analysis of the noncovalent interaction between the molecular hydrogen and protonated betaine molecule in the gas phase.
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