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Aptamer-Antibody Birecognized Sandwich SERRS Biosensor in Accurate and Rapid Identification of Intraoperative Parathyroid Hormone.
Anal Chem
January 2025
State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, College of Energy, Discipline of Intelligent Instrument and Equipment, Cancer Center and Department of Breast and Thyroid Surgery, Department of Ultrasound, Xiang'an Hospital of Xiamen University, School of Medicine, Laboratory Animal Center Xiamen University, Xiamen University, Xiamen 361005, China.
With the increasing incidence of thyroid cancer worldwide and the increasing demand for surgery, the risk of parathyroid injury is also increasing, which will lead to postoperative hypoparathyroidism (HP) and hypocalcemia. In order to improve the quality of life of patients after surgery, there is an urgent need to develop a novel platform that can identify the parathyroid gland immediately during surgery. The parathyroid gland promotes the increase of blood calcium concentration by secreting parathyroid hormone (PTH).
View Article and Find Full Text PDFThe use of Pleurotus ostreatus by-products for the preparation of a gel-like polysaccharide with bioactive properties.
Int J Biol Macromol
January 2025
Instituto de Pesquisa Pelé Pequeno Príncipe, 80240-020 Curitiba, PR, Brazil; Faculdades Pequeno Príncipe, 80230-020 Curitiba, PR, Brazil. Electronic address:
The mushroom Pleurotus ostreatus is widely produced in Brazil and its stipes are discarded before commercialization. In the present study, this agricultural fungal waste (mushroom stipes), was analyzed by preparing an aqueous extract and obtaining the polysaccharides by ethanol precipitation (POS-extract). The fraction presented 37 % of carbohydrates and small amounts of proteins and phenolic compounds.
View Article and Find Full Text PDFThe impact of a marine heatwave on the productivity and carbon budget of a NW Mediterranean seaweed forest.
Mar Pollut Bull
January 2025
Dipartimento di Biologia, Università di Pisa, CoNISMa, via Derna 1, 56126 Pisa, Italy.
Marine forests support coastal biodiversity and ecosystem functioning. Nonetheless, how their productivity and carbon uptake might be affected by extreme events, such as marine heatwaves (MHWs), is yet to be explored. We experimentally evaluated the changes in oxygen and carbon budgets of the benthic community formed by the fucoid Ericaria brachycarpa induced by the exposure to a MHW.
View Article and Find Full Text PDFDevelopment of a novel molecular probe for visualizing mesothelin on the tumor via positron emission tomography.
Eur J Nucl Med Mol Imaging
January 2025
Institute of Radiation Medicine, Fudan University, Xietu Road 2094, Shanghai, 200032, China.
Objectives: Mesothelin (MSLN) is an antigen that is overexpressed in various cancers, and its interaction with tumor-associated cancer antigen 125 plays a multifaceted role in tumor metastasis. The serum MSLN expression level can be detected using enzyme-linked immunosorbent assay; however, non-invasive visualization of its expression at the tumor site is currently lacking. Therefore, the aim of this study was to develop a molecular probe for imaging MSLN expression through positron emission tomography (PET).
View Article and Find Full Text PDFTemperature-Enhanced Purine Metabolism-Based Versatile SERS Platform for Rapid Clinical Pathogens Diagnosis and Drug-Resistant Assessment.
Anal Chem
January 2025
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
Label-free surface-enhanced Raman spectroscopy (SERS) combined with machine learning (ML) techniques presents a promising approach for rapid pathogen identification. Previous studies have demonstrated that purine degradation metabolites are the primary contributors to SERS spectra; however, generating these distinguishable spectra typically requires a long incubation time (>10 h) at room temperature. Moreover, the lack of attention to spectral variations between strains of the same bacterial species has limited the generalizability of ML models in real-world applications.
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