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Comparison of different fixatives effects in histochemical stainings of peripheral nerve tissue.
Cell Mol Biol (Noisy-le-grand)
January 2025
Harran University Faculty of Veterinary Medicine Department of Anatomy, Sanliurfa, Turkey.
A pathological condition in the peripheral nerve tissue, which provides the connection between the organism and the external environment, negatively affects the standard of living. The nerve tissue histotechnology is of serious importance both for scientific studies and for clinical diagnosis. The fixation, which is one of the leading procedures for histological examination of tissues, aims to preserve tissue morphology.
View Article and Find Full Text PDFDominant Expression of Chromogranin B in Pituitary Corticotrophs and Its Putative Role in Interaction With Secretogranin III.
J Histochem Cytochem
January 2025
Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, Fujisawa, Japan.
SummaryPrevious studies have suggested that chromogranin A (CgA) is a partner molecule of secretogranin III (SgIII). In mouse pituitary corticotroph-derived AtT-20 cells, SgIII plays a role in sorting CgA/hormone aggregates into secretory granules (SGs). Although CgA expression is equivocal, CgB is clearly detectable in the rat pituitary corticotrophs.
View Article and Find Full Text PDFMultiplexed and Multiomic Mass Spectrometry Imaging of MALDI-IHC Photocleavable Mass Tag Tissue Probes, N-Glycomics, and the Extracellular Matrisome from FFPE Tissue Sections.
Methods Mol Biol
December 2024
Department of Pharmacology & Immunology, Medical University of South Carolina, Charleston, SC, USA.
Article Synopsis
- Recent advancements in single-cell imaging have enhanced our understanding of how individual cells interact and influence disease progression.
- The described protocol utilizes MALDI-IHC with UV photocleavable mass tags to enable the simultaneous imaging of different cell types.
- This method integrates N-glycomic and ECM-targeted proteomic imaging from the same tissue section, offering a holistic view of the cellular and extracellular microenvironments.
Enhanced detection of distinct honeycomb-structured neuronal SMARCC2 cytobodies in Parkinson's Disease via Cyclic Heat-Induced Epitope Retrieval (CHIER).
PLoS One
December 2024
Department of Anatomy and Medical Imaging, University of Auckland, Auckland, New Zealand.
Antigen retrieval is crucial for immunohistochemistry, particularly in formalin-fixed paraffin-embedded brain tissue, where fixation causes extensive crosslinking that masks epitopes. Heat Induced Epitope Retrieval (HIER) reverses these crosslinks, improving access to nuclear and aggregated proteins. We introduce Cyclic Heat-Induced Epitope Retrieval (CHIER), an advanced technique that builds on HIER by incorporating repeated cycles of heating and cooling.
View Article and Find Full Text PDFOverview of a comparative analysis of microsatellite instability and standard mismatch repair protein-deficiency tests in a large cancer cohort.
Pathologie (Heidelb)
November 2024
Department of Pathology, Forensic and Insurance Medicine, Semmelweis University, Budapest, Hungary.
Article Synopsis
- Mismatch repair deficiency (dMMR) and microsatellite instability (MSI) are important genetic markers in various cancers, especially gastrointestinal and endometrial types, and can indicate responsiveness to immune checkpoint inhibitors (ICIs).
- In a study involving 1,306 cancer cases, dMMR was determined through immunohistochemistry (IHC) testing for specific proteins, while MSI was assessed using pentaplex PCR, revealing an overall MSI-high incidence of 12.1% compared to a dMMR incidence of 20.3%.
- A significant discrepancy of 19.3% was found between dMMR and MSI results, particularly noted with a 60.9% discrepancy in
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