Organic anion transport in polarized epithelia and macrophages has previously been studied by monitoring the efflux of fluorescent organic anion dyes from cells. We adapted this strategy to the study organic anion transport in lymphocytes. Cloned lymphoma cells and normal and activated human T cells were loaded with a membrane-impermeant, organic anion dye (Lucifer Yellow) by electroporation. Dye efflux in lymphocytes was rapid, energy-dependent, and inhibitable by organic anion transporter inhibitors. Dye efflux could not be attributed to the effects of electroporation. In addition, electroporated, dye-loaded T helper cells retained the ability to properly respond to specific antigen. Thus, dye loss occurred in viable, functionally competent cells. These experiments demonstrate that electroporation is an effective means of loading cells with Lucifer Yellow, and that lymphocytes possess organic anion transporters that are functionally similar to those previously described for secretory epithelia and macrophages.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0022-1759(92)90293-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chemiluminescence Resonance Energy Transfer for Targeted Photoactivation of Ion Channels in Living Cells.
Angew Chem Int Ed Engl
January 2025
Institute of Chemistry Chinese Academy of Sciences, Key Laboratory of Organic Solids, Zhongguancun North First Street 2, 100190, 100190, Beijing, CHINA.
The regulation of oxidative stress in living cells is essential for maintaining cellular processes and signal transduction. However, developing straightforward strategies to activate oxidative stress-sensitive membrane channels in situ poses significant challenges. In this study, we presented a chemiluminescence resonance energy transfer (CRET) system based on a conjugated oligomer, oligo(p-phenylenevinylene)-imidazole (OPV-Im), designed for the activation of transient receptor potential melastatin 2 (TRPM2) calcium channels in situ by superoxide anion (O2•-) without requiring external light sources.
View Article and Find Full Text PDFUnlocking Peak Efficiency in Anion-Exchange Membrane Electrolysis with Iridium-Infused Ni/NiP Heterojunction Electrocatalysts.
Small
January 2025
Advanced Materials Institute of Nano Convergence Engineering (BK21 FOUR), Dept. of Nano Convergence Engineering, Jeonbuk National University, Jeonju, Jeonbuk, 54896, Republic of Korea.
Developing cost-effective, highly efficient, and durable bifunctional electrocatalysts for water electrolysis remains a significant challenge. Nickel-based materials have shown promise as catalysts, but their efficiency in alkaline electrolytes is still lacking. Fascinatingly, Mott-Schottky catalysts can fine-tune electron density at interfaces, boosting intermediate adsorption and facilitating desorption to reduce the energy barrier.
View Article and Find Full Text PDFA "Flexible" Solvent Molecule Enabling High-Performance Lithium Metal Batteries.
Angew Chem Int Ed Engl
January 2025
Southern University of Science and Technology, Department of Mechanical and Energy Engineering, CHINA.
Localized high-concentration electrolytes (LHCEs) exhibit good performance in lithium metal batteries. However, understanding how the intermolecular interactions between solvents and diluents regulate the solvation structure and interfacial layer structure remains limited. Here, we reported an LHCE in which strong hydrogen bonding between diluents and solvents alters the conformation and polarity of "flexible" solvent molecules, thereby effectively regulating the solvation structure of Li+ ion and promoting the formation of robust electrode interfaces.
View Article and Find Full Text PDFUnusual Caesium Monensinate A: Crystallographic Evidence for the Formation of Dinuclear Coordination Species in the Solid State.
Chemistry
January 2025
NDSU: North Dakota State University, Department of Chemistry and Biochemistry, UNITED STATES OF AMERICA.
The polyether ionophore monensin A (MonH), applied as silver monensinate, reacts with caesium cations to form a dinuclear complex [Mon2Cs2] the structure of which has been solved by single-crystal X-ray diffraction. Two Cs+ ions are located in the hydrophilic cage of two ligand anions, achieving coordination number eight. In addition, the metal cations are bridged by two functional groups of monensinate A, completing the inner tenfold coordination sphere.
View Article and Find Full Text PDFClinical Evaluation of Drug-Drug Interactions with Obeldesivir, an Orally Administered Antiviral Agent.
Clin Pharmacol Ther
January 2025
Gilead Sciences, Inc., Foster City, California, USA.
Obeldesivir is an oral nucleoside analog prodrug inhibitor of SARS-CoV-2 RNA-dependent RNA polymerase and other viral polymerases. Here, two Phase I studies evaluated potential drug-drug interactions between obeldesivir and substrates or inhibitors of cytochrome P450 and drug transporters in healthy participants. When obeldesivir was tested as a precipitant, pharmacokinetic parameter point estimates for midazolam (CYP3A4 inhibition/induction), caffeine (CYP1A2 inhibition), and metformin (organic cation transporter 1 inhibition) exposures were within 80-125% no-effect bounds representing the interval within which a systemic exposure change does not warrant clinical action based on EMA/FDA guidance.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!