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Species of the Intermountain Western United States: A Chemotaxonomic Investigation Based on Essential Oil Compositions.
Plants (Basel)
January 2025
Department of Crop and Soil Science, Oregon State University, Ontario, OR 97914, USA.
is a genus of 98 species, widely distributed in western North America. This work presents a chemometric analysis of the essential oils of seven species of (, var. , , , , , and var.
View Article and Find Full Text PDFLeaf Essential Oil Compositions and Enantiomeric Distributions of Monoterpenoids in Species: , , , , and .
Molecules
January 2025
Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA.
Members of the genus are well known for their medicinal properties, which can be attributed to their essential oils. In this work, we have examined the leaf essential oils of five understudied species collected from various locations in western North America. The essential oils were obtained by hydrodistillation and analyzed by gas chromatographic methods, including enantioselective gas chromatography.
View Article and Find Full Text PDFFluorinated Macrocycle for Adsorptive Separation and Chromatographic Separation of Toluene and Methylcyclohexane.
Chemistry
January 2025
Tianjin Normal University, Chemistry, No393 west Binshui Road, Tianjin, CHINA.
Achieving the adsorptive separation and chromatographic separation of industrially the important chemicals toluene and methylcyclohexane using the same material is a highly desirable goal. We have successfully accomplished this using a fluorinated macrocycle tetrafluoroterphen[3]arene (4FTP3), which was synthesized and used for gas chromatographic separation in our previous work. The macrocycle 4FTP3 permitted the adsorptive separation of toluene from a toluene/methylcyclohexane mixture (1:1, v/v) with a purity of 99.
View Article and Find Full Text PDFA Protocol for GC-MS Profiling of Chiral Secondary Amino Acids.
Methods Mol Biol
January 2025
Laboratory of Analytical Biochemistry & Metabolomics, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.
A simple analytical workflow is described for gas chromatographic-mass spectrometric (GC-MS)-based chiral profiling of secondary amino acids (AAs) in biological matrices. The sample preparation is carried out directly in aqueous biological sample extracts and involves in situ heptafluorobutyl chloroformate (HFBCF) derivatization-liquid-liquid microextraction of nonpolar products into hexane phase followed by subsequent formation of the corresponding methylamides from the HFB esters by direct treatment with methylamine reagent solution. The (O, N) HFB-butoxycarbonyl-methylamide AA products (HFBOC-MA) are separated on a Chirasil-L-Val capillary column and quantitatively measured by GC-MS operated in selected ion monitoring (SIM) mode.
View Article and Find Full Text PDFSpecific activity of mouse liver desaturases and elongases: Time course effects using n-3 and n-6 PUFA substrates and inhibitory responses of delta-6 desaturase.
Biochim Biophys Acta Mol Cell Biol Lipids
January 2025
Department of Nutritional Sciences, Temerty Faculty of Medicine, University of Toronto, Toronto, Canada.
The synthesis of n-3 and n-6 polyunsaturated acids (PUFAs) is associated with physiological functions in mammals, being catalyzed by Δ-5D and Δ-6D desaturases and elongases Elovl-2 and Elovl-5. In this context, we aimed to study the chief kinetic features of PUFA liver anabolism, looking upon (i) the time-dependency for the specific activity of Δ-6D, Δ-5D, Elovl2, Elovl2/5 and Elovl5, using n-3 and n-6 precursors between 0 and 240 min ex vivo in mouse liver.; and (ii) the specific activity-substrate (α-linolenic acid; ALA) concentration responses of Δ-6D in the absence and presence of linoleic acid (LA), arachidonic acid (ARA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), an enzyme regarded as the rate-limiting step in PUFA anabolism.
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