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Magnetic Field-Accelerated Nonthermal Plasma Digestion for Field Pretreatment and Determination of Heavy Metals in Biological Samples.
Anal Chem
January 2025
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
Field analysis of heavy metals in biological samples is essential for assessing their potential threats to human health. The development of portable pretreatment and detection devices is crucial to address this challenge. Herein, a magnetic field-accelerated nonthermal plasma digestion device using dielectric barrier discharge (DBD) is designed for the rapid and environmentally friendly pretreatment of biological samples and subsequently combined with point discharge-optical emission spectrometry (PD-OES) for sensitive determination of heavy metals.
View Article and Find Full Text PDFPediatric simple triage score: A simplified approach for triaging pediatric patients with fever in the emergency department.
Turk J Emerg Med
January 2025
Department of Emergency Medicine, Travancore Medical College Hospital, Kollam, Kerala, India.
Introduction: The initial 24-h period following admission to a hospital holds profound significance for pediatric patients, representing a critical window where proactive interventions can substantially influence outcomes. We devised a simple triage system, pediatric simple triage score (PSTS), to see whether rapid triage of sick pediatric patients with fever can be done using the new triage system in the emergency department (ED) to predict hospital admission.
Methods: This was a prospective observational study, conducted at the department of emergency medicine of a tertiary care teaching hospital in southern India.
Rapid and accurate demultiplexing of direct RNA nanopore sequencing datasets with SeqTagger.
Genome Res
January 2025
Center for Genomic Research (CRG), Universitat Pompeu Fabra (UPF), ICREA
Nanopore direct RNA sequencing (DRS) enables direct measurement of RNA molecules, including their native RNA modifications, without prior conversion to cDNA. However, commercial methods for molecular barcoding of multiple DRS samples are lacking, and community-driven efforts, such as DeePlexiCon, are not compatible with newer RNA chemistry flowcells and the latest-generation GPU cards. To overcome these limitations, we introduce SeqTagger, a rapid and robust method that can demultiplex direct RNA sequencing datasets with 99% precision and 95% recall.
View Article and Find Full Text PDFDual-OR Logic-Gated Lateral Flow Strip Assay Based on Colorimetric-Fluorescence Dual Indication for Screening of HPV16/18 in Multiple Scenarios.
Anal Chem
January 2025
Center of Clinical Laboratory Medicine, Zhongda Hospital, Medical School of Southeast University, Nanjing 210009, Jiangsu, China.
The incidence of cervical cancer continues to rise in underdeveloped regions due to low human papillomavirus (HPV) vaccination rates and inadequate screening systems. To achieve convenient, rapid, and accurate detection of HPV, we developed a three-wire lateral flow strip assay system based on dual-OR logic gates for rapid and simultaneous detection of HPV subtypes 16 and 18 in a single test. The system combines three-branch-catalytic hairpin assembly (TCHA)-mediated signal amplification with simple OR logic gate-based signal output to improve detection rates while enabling HPV 16/18 subtype identification.
View Article and Find Full Text PDFSensitive fluorescent detection of SARS-CoV-2 RNA using an enzymatic-based method.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
CICS-UBI - Health Sciences Research Centre University of Beira Interior Covilhã Portugal; RISE-Health, Departamento de Química, Faculdade de Ciências, Universidade da Beira Interior, Rua Marquês d'Ávila e Bolama 6201-001 Covilhã, Portugal; Departamento de Química, Universidade da Beira Interior, Rua Marquês de Ávila e Bolama 6201-001 Covilhã, Portugal. Electronic address:
Rapid, quantitative, and sensitive detection of viral oligonucleotides can help to diagnose the infection before symptoms occur, monitor disease progression, and identify viral subtypes. A one-pot, simple, rapid hairpin-mediated nicking enzymatic signal amplification (HNESA) method was previously developed for nucleic acids detection. In the present work, this method was applied for the detection of SARS-CoV-2 RNA by designing an assistant probe (AP) that contains the complementary sequence for the target, the sequence of hybridization with the loop region of the molecular beacon (MB), and the recognition site of the nicking endonuclease Nt.
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