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Unusual depolymerization mechanism of Poly(ethylene terephthalate) by hydrolase 202.
Chemosphere
January 2025
Environment Research Institute, Shandong University, Qingdao, 266237, PR China.
Polyethylene terephthalate (PET) waste significantly contributes to the global plastic crisis, but enzymatic conversion has become an efficient and environmentally friendly strategy to combat it. Therefore, this study explored the Re-face selective depolymerization mechanisms of a novel PET-degradation peptidase, hydrolase 202. Theoretical calculations revealed that the first step, a catalytic triad-assisted nucleophilic attack, is the rate-determining step.
View Article and Find Full Text PDFUncovering the substrate of olefin synthase loading domains in cyanobacteria sp. strain PCC 7002.
RSC Chem Biol
January 2025
Department of Chemical and Biological Engineering, University of Wisconsin - Madison Madison Wisconsin 53706 USA
Cyanobacteria are widespread, photosynthetic, gram-negative bacteria that generate numerous bioactive secondary metabolites complex biosynthetic enzymatic machinery. The model cyanobacterium sp. strain PCC 7002, hereafter referred to as PCC 7002, contains a type I polyketide synthase (PKS), termed olefin synthase (OlsWT), that synthesizes 1-nonadecene and 1,14-nonadecadiene: α-olefins that are important for growth at low temperatures.
View Article and Find Full Text PDFProduction of free fatty acids by enzymatic hydrolysis of residual frying oil using non-commercial lipases from Aspergillus niger.
An Acad Bras Cienc
January 2025
Universidade Federal de Sergipe, Departamento de Engenharia Química - DEQ, Laboratório de Laboratório de Biotecnologia Ambiental (LABAM), Campus São Cristóvão, Rodovia Marechal Rondon, s/n, Rosa Elze, 49100-000 São Cristóvão, SE, Brazil.
Lipases are enzymes that have an important role in the industry for their wide use, giving rise to a great interest in industrial bioprocesses due to their versatility. One of the applications is the enzymatic hydrolysis of waste oils. This work consists of evaluating the production of lipases using several concentrations of residual frying oil (RFO) and different pHs, through ANOVA analysis.
View Article and Find Full Text PDFTransforming Feather Meal Into a High-Performance Feed for Broilers.
Vet Med Sci
January 2025
Department of Industrial Management, Faculty of Humanities, University of Tehran, Kish International Campus, Tehran, Iran.
Background: The poultry industry faces challenges with the high cost and environmental impact of Soybean meal. Feather meal, a byproduct with low digestibility due to its keratin content, is a potential alternative. Recent biotechnological advances, including enzymatic and bacterial hydrolysis, have enhanced its digestibility and nutritional value.
View Article and Find Full Text PDFPrecision biotic as an effective replacement of hydrolyzed yeast and butyrate in antibiotic free diets of broiler chickens raised under field conditions.
Poult Sci
December 2024
dsm-firmenich, Kaiseraugst, Switzerland.
A total of 1,436,000 Ross 380 AP broiler chicks were included in the experiment, which was conducted in two cycles with 20 houses per cycle and 35,900 birds per house. The objective was to evaluate, under field conditions, the impact of a precision biotic (PB) on the growth performance and cecal microbiome of broiler chickens, in comparison to enzymatically hydrolyzed yeast (EHY) and butyrate (BT) in an antibiotic-free diet. Each cycle consisted of six (6) houses under PB supplementation, and 14 houses under the regular dietary program used by the integration.
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