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Anticoagulant Activity of the Polysaccharide Fromgonad of Abalone Ino: The Role of Conjugate Protein.
Foods
December 2024
National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Few studies are concerned with the effect of the conjugat protein on the bioactivities of the abalone gonad polysaccharide (AGP). In this study, a series of treatments, including raw material (female and male) defatting, extraction temperature (25-121 °C), proteolysis, ultrafiltration, and ethanol precipitation, was conducted to investigate the role of the conjugate protein on AGP anticoagulant activity. All AGP extracts significantly prolonged activated partial thromboplastin time (APTT) and thrombin time (TT).
View Article and Find Full Text PDFStructure and strain specificity for polysaccharides from king oyster mushroom (Pleurotus eryngii) fruiting bodies.
Int J Biol Macromol
January 2025
Department of Crop Production, Czech University of Life Sciences Prague, Czech Republic.
King oyster mushroom Pleurotus eryngii is cultivated worldwide for culinary and to improve human health. However, the potential of some Mediterranean representatives of this species is still not evaluated. This work focuses on the study of polysaccharides from fruiting bodies of two Tunisian strains, P.
View Article and Find Full Text PDFLC-MS/MS analysis of surface and lysate N-glycans of CHO-K1 cells: Structure, relative quantity, and absolute quantity.
J Chromatogr B Analyt Technol Biomed Life Sci
December 2024
Biotherapeutics and Glycomics Laboratory, College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea; Department of Global Innovative Drugs, Graduate School of Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea. Electronic address:
Chinese hamster ovary (CHO)-K1 cells are widely used in biomedical research relevant to cancer, toxicity screening, and viruses, as well as in the production of recombinant proteins for biopharmaceuticals. In this study, liquid chromatography (LC)-electrospray ionization (ESI)-higher energy collisional dissociation (HCD)-tandem mass spectrometry (MS/MS) was used to characterize the surface and lysate N-glycans of CHO-K1 cells and analyze their structures. The relative quantity (%) of each N-glycan and absolute quantity (pmol) of total N-glycans were also obtained.
View Article and Find Full Text PDFIn-Depth Analysis of Tissue Phosphoproteomics with TiO Enrichment and Fractionation with High-pH Reversed-Phase Chromatography.
Methods Mol Biol
December 2024
Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Protein phosphorylation is an important post-translational modification that regulates almost all cellular processes, such as cellular metabolism, growth, differentiation, signal transduction, and gene regulation. Mass spectrometry, which acts as an automated and sensitive method, enables global analysis of protein phosphorylation. However, several technical challenges need to be addressed when analyzing protein phosphorylation in a global manner.
View Article and Find Full Text PDFUmbrella Sampling MD Simulations for Retention Prediction in Peptide Reversed-phase Liquid Chromatography.
Anal Chem
January 2025
Department of Chemistry, The University of Western Ontario, London, Ontario N6A 5B7, Canada.
Reversed-phase liquid chromatography (RPLC) is an essential tool for separating complex mixtures such as proteolytic digests in bottom-up proteomics. There is growing interest in methods that can predict the RPLC retention behavior of peptides and other analytes. Already, existing algorithms provide excellent performance based on empirical rules or large sets of RPLC training data.
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