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Capillary Wave-Assisted Colloidal Assembly.
Langmuir
January 2025
Cain Department of Chemical Engineering, Louisiana State University, Baton Rouge, Louisiana 70803, United States.
The self-assembly of nanoparticle colloids into large-area monolayers with long-range order is a grand challenge in nanotechnology. Using acoustic energy, i.e.
View Article and Find Full Text PDFRapid and accurate demultiplexing of direct RNA nanopore sequencing datasets with SeqTagger.
Genome Res
January 2025
Center for Genomic Research (CRG), Universitat Pompeu Fabra (UPF), ICREA
Nanopore direct RNA sequencing (DRS) enables direct measurement of RNA molecules, including their native RNA modifications, without prior conversion to cDNA. However, commercial methods for molecular barcoding of multiple DRS samples are lacking, and community-driven efforts, such as DeePlexiCon, are not compatible with newer RNA chemistry flowcells and the latest-generation GPU cards. To overcome these limitations, we introduce SeqTagger, a rapid and robust method that can demultiplex direct RNA sequencing datasets with 99% precision and 95% recall.
View Article and Find Full Text PDFFacile preparation of iridium-based AIE polymer dots for sensitive electrochemiluminescence immunoassay of CD44 protein.
Anal Chim Acta
March 2025
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China; Department of Chemistry, Sungkyunkwan University, Suwon, 16419, Republic of Korea. Electronic address:
The development of aggregation-induced emission (AIE) luminophores is a fascinating and promising topic in electrochemiluminescence (ECL) bioanalysis. Herein, the AIE-active but water-insoluble [Ir(bt)₂(acac)] (bt = 2-phenylbenzothiazole, acac = acetylacetonate) was encapsulated within poly(styrene-maleic anhydride) (PSMA) using a simple nanoprecipitation method. This encapsulation strategy could effectively limit the free motion of Ir(bt)₂(acac) and trigger the aggregation-induced electrochemiluminescence (AIECL) effect.
View Article and Find Full Text PDFSCoT and ISSR based conservation genetics of Calligonum polygonoides Linn.: An endangered species of Thar desert.
Gene
January 2025
Department of Botany, Mohanlal Sukhadia University, Udaipur 313001, Rajasthan, India. Electronic address:
Calligonum polygonoides, an endangered species of desert due to poor regeneration and overexploitation, which requires immediate conservation attention. Genetic diversity analysis is crucial for effective conservation and management initiatives, for elite genotypes. Therefore, in the present study, SCoT (start codon target) and ISSR (inter simple sequence repeat) markers were used to investigate the genetic variability in 120 individuals of Calligonum polygonoides.
View Article and Find Full Text PDFCofactor-directed co-immobilization of dual-enzyme on functionalized montmorillonite with enhanced catalytic performance.
Int J Biol Macromol
January 2025
School of Environment and Resource, Key Laboratory of Solid Waste Treatment and Resource Recycle of Ministry of Education, Southwest University of Science and Technology, Mianyang, Sichuan 621010, China.
Recently, multi-enzyme cascade catalysis has attracted increasing attention due to the advantages of integrating multiple enzymes, few side reactions and high catalytic efficiency. Herein, a novel dual-enzyme cascade system (GOx-FMt-HRP) was developed through cofactor-directed orientational co-immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP) onto functional montmorillonite (FMt). The presented method realizes the reconstitution of cofactors and apo-enzymes (enzymes without cofactors), which enables enzymes to be immobilized in specific orientations on the support, thereby effectively reducing changes in their conformation.
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