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Cell Identification, Characterization, and Documentation for Use in the Production of Biological Products.
Arch Razi Inst
June 2024
Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), P.O. Box 31975-148, Karaj, Iran.
There is always a concern about the quality of cell-based products in terms of the contamination of the cells and their lack of efficiency. Therefore, it is of prime importance to ensure these cells' identity, purity, efficacy, and suitability for the production of biological products and diagnostic uses. Hence, cells must be identified, evaluated, documented, and stored to be used consistently and efficiently.
View Article and Find Full Text PDFMobile unidirectional airflow device reduces air contamination in an intravitreal outpatient clean room setting.
Int Ophthalmol
December 2024
Department of Medicine Surgical, Section of Ophthalmology, University of Perugia, S. Maria Della Misericordia Hospital, 06156, Perugia, Italy.
Purpose: To evaluate and compare the mycobacterial load using a mobile laminar airflow (LAF) device in an IVI-dedicated outpatient clean room (OCR) without ventilation systems and in a hospital-based operating theatre (HOT).
Methods: This case-control study was conducted in 2 different settings: OCR and HOT during a series of intravitreal injections (IVIs). The Air Microbial analysis was performed using a Surface Air System instrument at three different moments during the IVI sessions in both settings: at the operative site (OS), four meters from the OS (DOS) and in the disinfection room (DR).
pseudo-outbreak due to laboratory cross-contamination: A molecular epidemiology outbreak investigation.
Can Commun Dis Rep
December 2024
Immunopathology Axis, Centre de recherche du Centre hospitalier de l'Université de Montréal, Montréal, QC.
Background: Mycobacterial culture is routinely performed to diagnose tuberculosis (TB) in Canada. Globally, meta-analyses suggest that up to 2% of positive cultures are falsely positive for due to laboratory cross-contamination. Five patients from distinct clinical institutions in Montréal were diagnosed with culture-positive TB as their clinical samples were processed in a centralized mycobacteria laboratory.
View Article and Find Full Text PDFSputum culture contamination and associated characteristics in a diagnostic clinical trial, Papua New Guinea.
Public Health Action
December 2024
Burnet Institute, Melbourne, Australia.
Setting: Papua New Guinea is a high-burden multidrug-resistant TB (MDR/RR-TB) country that reports low rates of bacteriological confirmation. Sputum drug susceptibility testing for second-line drugs is important. Access to culture is limited.
View Article and Find Full Text PDFDiscovery of subsp. Lytic Phages with Extensive Host Range Across Rapid- and Slow-Growing Pathogenic Mycobacterial Species.
Antibiotics (Basel)
October 2024
Department of Microbiology, College of Science, Oregon State University, Corvallis, OR 97331, USA.
Background/objectives: Developing interventions for Johne's disease, which focuses on controlling subsp. (MAP) in contaminated environments by treating infected cows and preventing transmission from diseased animals, is a critical priority. Bacteriophage (phage) therapy, an emerging biological intervention, offers a promising alternative for the treatment and management of MAP infections.
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