Attempts were made to examine the input of some ecological and physiological factors into the fluctuation of the lymphocyte transformation assay results. The spontaneous and PHA-induced 3H-thymidine uptake in lymphocyte cultures of adult healthy persons and healthy or rheumatic fever children fluctuated at different seasons of the year with the maximum at summer. The activity of cultured women's lymphocytes depended on the phases of menstrual cycle of donors.
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