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Consolidated bioprocessing of cassava starch into butyric acid and butanol by engineered Clostridium butyricum SCUT 620.
Bioresour Technol
February 2025
School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China; Guangdong Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou 510006, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510006, China. Electronic address:
Clostridium butyricum SCUT 620, a promising biorefinery chassis, has been demonstrated to efficiently utilize monosaccharides, disaccharides, and polysaccharides for butyric acid production. However, the absence of genetic manipulation tools has restricted its further development and application. For the first time, an efficient electroporation method for C.
View Article and Find Full Text PDFOptimization of ultrasound-mediated DNA transfer for bacteria and preservation of frozen competent cells.
Microbiol Spectr
November 2024
Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, China.
Unlabelled: The transformation of DNA into cells is the basis of molecular biology. Commonly employed techniques include heat shock transformation, electro-transformation, conjugation, transduction, and protoplast fusion. Recently, ultrasonic transformation technology has been developed to transfer DNA into competent cells.
View Article and Find Full Text PDFpoxtA amplification and mutations in 23S rRNA confer enhanced linezolid resistance in Enterococcus faecalis.
J Antimicrob Chemother
December 2024
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
Objectives: This study aimed to explore the evolutionary patterns and resistance mechanisms of an Enterococcus faecalis strain harbouring poxtA under linezolid exposure.
Methods: A poxtA-carrying E. faecalis electrotransformant DJH702 with a linezolid minimum inhibitory concentration of 4 mg/L was exposed to increasing concentrations of linezolid (8-64 mg/L).
Emergence of plasmid-borne (X4) resistance gene in clinical isolate of eravacycline- and omadacycline-resistant ST485.
Microbiol Spectr
September 2024
Jinan Microecological Biomedicine Shandong Laboratory, Jinan, China.
Article Synopsis
- - Omadacycline and eravacycline are new tetracycline antibiotics being used against Gram-negative bacteria, but resistance has been reported due to tetracycline-inactivating enzymes.
- - A resistance strain with the mobile tigecycline resistance gene (X4) was isolated from a patient in Zhejiang, China, showing high resistance levels to eravacycline and omadacycline.
- - The study emphasizes the role of the (X4) gene on a plasmid in contributing to antibiotic resistance and highlights the need for ongoing monitoring to control its spread in China.
The elimination of two restriction enzyme genes allows for electroporation-based transformation and CRISPR-Cas9-based base editing in the non-competent Gram-negative bacterium sp. Tol 5.
Appl Environ Microbiol
June 2024
Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Nagoya, Japan.
Environmental isolates are promising candidates for new chassis of synthetic biology because of their inherent capabilities, which include efficiently converting a wide range of substrates into valuable products and resilience to environmental stresses; however, many remain genetically intractable and unamenable to established genetic tools tailored for model bacteria. sp. Tol 5, an environmentally isolated Gram-negative bacterium, possesses intriguing properties for use in synthetic biology applications.
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