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Establishment of a rapid method for the detection of canis based on recombinase-mediated thermostable nucleic acid amplification technology.
Front Cell Infect Microbiol
January 2025
The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China.
Objective: To establish a rapid detection method for canine using recombinase-aided amplification (RAA) technology.
Methods: The outer membrane protein 25 gene fragment (Omp25) of canis was targeted. Primers and fluorescent probes were designed and synthesized, and recombinant plasmids were constructed as standards.
Effects of probiotic coating and vacuum packaging on the microbial safety and quality of fresh-cut cantaloupe stored at different temperatures.
J Food Sci
January 2025
Department of Food Science & Technology, Faculty of Science, National University of Singapore, Singapore, Singapore.
Fresh-cut cantaloupes are highly susceptible to contamination by foodborne pathogens and spoilage-causing microorganisms. This study evaluated the efficacy of a probiotic coating produced by fermenting Lactiplantibacillus plantarum 299 V in pomelo peel extract in combination with vacuum packaging in controlling the microbial loads of fresh-cut cantaloupe during storage. As temperature abuse is common in transportation and at retail sale of such products in many countries, we evaluated their efficacy at different temperatures.
View Article and Find Full Text PDFMicrobial safety of black soldier fly larvae (Hermetia illucens) reared on food waste streams.
Waste Manag
January 2025
Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Brisbane, Queensland 4072, Australia; School of Agriculture and Food Sustainability, Faculty of Science, University of Queensland, Brisbane, Queensland 4072, Australia.
Black soldier fly larvae (BSFL) can valorise different organic matter and yield a product of high nutritional value. The lack of knowledge about the microbial safety of BSFL grown on different organic waste streams influences the commercialisation of BSFL as stockfeed ingredient. This study evaluates the microbial safety of BSFL grown on five different commercial food waste streams collected from two commercial production facilities.
View Article and Find Full Text PDFSpent coffee ground disrupts Listeria monocytogenes biofilm formation through inhibition of motility and adhesion via quorum sensing regulation.
Int J Food Microbiol
January 2025
Department of Food Science and Biotechnology, Dongguk University, Goyang 10326, Republic of Korea. Electronic address:
Spent coffee grounds (SCGs) have been explored for use as various bioresources, such as biofuels, and are known to possess biological functions, including antioxidant activity. However, the antibiofilm properties of SCGs against pathogenic bacteria have not been fully investigated. Therefore, this study aimed to highlight the inhibitory effects of SCG extract (SCGE) on biofilm formation in Listeria monocytogenes and investigated the underlying mechanisms.
View Article and Find Full Text PDFA Dual-Mode Colorimetric and Fluorescence Biosensor Based on a Nucleic Acid Multiplexing Platform for the Detection of .
Anal Chem
January 2025
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.
() is one of the most prevalent threats, capable of inducing diverse illnesses and presenting a serious threat to public health. Herein, we demonstrate a novel dual-mode colorimetric/fluorescence biosensor based on the xponential mplification reaction and trand isplacement eactions (EASDR), which has multiplexing capability that significantly promotes the anchoring and trapping of Pt nanoparticles (Pt NPs) and fluorescent dyes for sensitive detection of (). The method works by targeting specific bacteria with aptamers and promoting repeated EASDR to affect the immobilization of Pt NPs and fluorescent dyes in the orifice plate, which could produce changes in fluorescence and colorimetric signals.
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