AI Article Synopsis
- Methods for estimating plasma and urine alpha-amino nitrogen using the colorimetric ninhydrin reaction are outlined.
- In the urine method, ammonia and urea are separated using a quick ion-exchange resin process.
- Two estimation methods are provided: one is simple but uses cyanide, while the other avoids cyanide, with both showing normal values consistent with the gasometric ninhydrin method.
Article Abstract
Methods are described for the estimation of plasma and urine alpha-amino nitrogen by the colorimetric ninhydrin reaction; in the method for urine, both ammonia and urea are separated by a rapid ion-exchange resin procedure. Two methods of estimation are given; one has the advantage of great simplicity, but involves the use of cyanide; in the other, cyanide is eliminated. Normal values are reported; they correspond well with those obtained by the gasometric ninhydrin method.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC480706 | PMC |
| http://dx.doi.org/10.1136/jcp.17.2.150 | DOI Listing |
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