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Dual-Target Additively Manufactured Electrochemical Sensor for the Multiplexed Detection of Protein A29 and DNA of Human Monkeypox Virus.
ACS Omega
July 2024
Laboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, Brazil.
Herein, we present the first 3D-printed electrochemical portable biodevice for the detection of monkeypox virus (MKPV). The electrochemical device consists of two biosensors: an immunosensor and a genosensor specifically designed for the detection of the protein A29 and a target DNA of MKPV, respectively. The electrodes were manufactured using lab-made ultraflexible conductive filaments composed of carbon black, recycled PLA from coffee pods, and castor oil as a plasticizer.
View Article and Find Full Text PDFCarbon dioxide fluxes in Alpine grasslands at the Nivolet Plain, Gran Paradiso National Park, Italy 2017-2023.
Sci Data
June 2024
Istituto di Geoscienze e Georisorse, IGG-CNR, Via Moruzzi 1, 56124, Pisa, Italy.
We introduce a georeferenced dataset of Net Ecosystem Exchange (NEE), Ecosystem Respiration (ER) and meteo-climatic variables (air and soil temperature, air relative humidity, soil volumetric water content, pressure, and solar irradiance) collected at the Nivolet Plain in Gran Paradiso National Park (GPNP), western Italian Alps, from 2017 to 2023. NEE and ER are derived by measuring the temporal variation of CO concentration obtained by the enclosed chamber method. We used a customised portable non-steady-state dynamic flux chamber, paired with an InfraRed Gas Analyser (IRGA) and a portable weather station, measuring CO fluxes at a number of points (around 20 per site and per day) within five different sites during the snow-free season (June to October).
View Article and Find Full Text PDFElectroanalytical Overview: The Sensing of Mesalamine (5-Aminosalicylic Acid).
ACS Meas Sci Au
February 2024
Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, United Kingdom.
Mesalamine, known as 5-aminosalicylic acid, is a medication used primarily in the treatment of inflammatory bowel disease, including ulcerative colitis and Crohn's disease. 5-Aminosalicylic acid can be measured using various benchtop laboratory techniques which involve liquid chromatography-mass spectroscopy, but these are sophisticated and large, meaning that they cannot be used on-site because transportation of the samples, chemicals, and physical and biological reactions can potentially occur, which can affect the sample's composition and potentially result in inaccurate results. An alternative approach is the use of electrochemical based sensing platforms which has the advantages of portability, cost-efficiency, facile miniaturization, and rapid analysis while nonetheless providing sensitivity and selectivity.
View Article and Find Full Text PDFElectroanalytical overview: the sensing of carbendazim.
Anal Methods
September 2023
Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK.
Carbendazim is a broad-spectrum systemic fungicide that is used to control various fungal diseases in agriculture, horticulture, and forestry. Carbendazim is also used in post-harvest applications to prevent fungal growth on fruits and vegetables during storage and transportation. Carbendazim is regulated in many countries and banned in others, thus, there is a need for the sensing of carbendazim to ensure that high levels are avoided which can result in potential health risks.
View Article and Find Full Text PDFElectroanalytical overview: the sensing of hydroxylamine.
Anal Methods
June 2023
Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK.
One of the principal raw ingredients used in the manufacturing of pharmaceuticals, nuclear fuel, and semiconductors is hydroxylamine, a mutagenic and carcinogenic substance, ranking high on the list of environmental contaminants. Electrochemical methods for monitoring hydroxylamine have the advantage of being portable, quick, affordable, simple, sensitive, and selective enough to maintain adequate constraints in contrast with conventional yet laboratory based quantification methods. This review outlines the most recent advancements in electroanalysis directed toward the sensing of hydroxylamine.
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