Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Development and Characterization of a Polyvalent Polyclonal Antibody as a Common Capture Antibody for the Detection of Enterotoxigenic Escherichia coli in a Sandwich ELISA.
Curr Microbiol
March 2025
Centro de Investigación en Biotecnología Aplicada del Instituto Politecnico Nacional, Ex-Hacienda San Juan Molino Carretera Estatal Tecuexcomac-Tepetitla Km 1.5, 90700, Tlaxcala, Mexico.
Due to its low cost and simplicity, the sandwich enzyme-linked immunosorbent assay (sELISA) is a traditional technique for identifying foodborne pathogens. However, most sELISAs are designed for single foodborne pathogen detection using two specific antibodies, which capture and detect the target bacteria. This study aimed to produce and characterize a common capture polyclonal antibody for Enterotoxigenic Escherichia coli (ETEC), Salmonella Typhimurium, and Shigella flexneri (S.
View Article and Find Full Text PDFAn antibody-toxin conjugate targeting CD47 linked to the bacterial toxin listeriolysin O for cancer immunotherapy.
Nat Cancer
February 2025
Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Antigen-presenting cells phagocytose tumor cells and subsequently cross-present tumor-derived antigens. However, these processes are impeded by phagocytosis checkpoints and inefficient cytosolic transport of antigenic peptides from phagolysosomes. Here, using a microbial-inspired strategy, we engineered an antibody-toxin conjugate (ATC) that targets the 'don't eat me' signal CD47 linked to the bacterial toxin listeriolysin O from the intracellular bacterium Listeria monocytogenes via a cleavable linker (CD47-LLO).
View Article and Find Full Text PDFImmunogenicity of culture filtrated proteins and whole-cell killed formalin of to induced cellular immune response .
Open Vet J
December 2024
Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Tikrit, Tikrit, Iraq.
Background: (LM) is a life-threatening bacterium affecting many individuals worldwide, including elderly people, pregnant women, and immune-deficient patients.
Aim: Whole-cell killed formalin of LM antigens (WKLMAgs) and culture filtrated proteins (LCFPs) against challenge-attenuated LM after two booster doses (0 and 14 days) of immunization act as an antigen activating a high level of IgG3, IgM, CXCL2, and IL-1 beta.
Methods: Forty male rats were randomly assigned to four groups.
One health investigation following a cluster of Crimean-Congo haemorrhagic fever, North Macedonia, July to November 2023.
Euro Surveill
January 2025
Institute of Biology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
BackgroundCrimean-Congo haemorrhagic fever (CCHF) is a severe illness characterised by fever, bleeding and high case-fatality rates. The disease is caused by CCHF virus (CCHFV), transmitted by ticks and infectious body fluids and tissues.AimAfter CCHF was diagnosed in three persons in 2023, we aimed to investigate the presence of antibodies against CCHFV in healthcare workers (HCW), sheep and goats, and of CCHFV in ticks, in an area in North Macedonia and characterise virus strains.
View Article and Find Full Text PDFDevelopment of a colloidal gold immunochromatographic assay utilizing dual-antibody sandwich method for detecting .
Front Microbiol
January 2025
College Food Science and Light Industry, Nanjing Tech University, Nanjing, China.
A colloidal gold immunochromatographic assay (ICA) based on a dual-antibody sandwich method was developed for the rapid and convenient detection of () antigens in the early stages of infection. Monoclonal antibodies designed as 5B3 targeting the conserved region of 56 kDa outer membrane protein in various strains of were generated through cell fusion and screening techniques and combined with previously prepared polyclonal antibodies as detection antibodies to establish the ICA. Colloidal gold and polyclonal antibody-colloidal gold complexes were synthesized under optimized conditions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!