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Bioactivities of secondary metabolites of two actinomycetes Streptomyces parvulus GloL3, and Streptomyces lienomycini SK5, endophytes of two Indian medicinal herbs- Globba marantina L. and Selaginella kraussiana (Kunze) A. Braun.
Braz J Microbiol
January 2025
Microbiology and Microbial Biotechnology Laboratory, Department of Botany and Forestry, Vidyasagar University, 721102, Midnapore, West Bengal, India.
Endophytic actinomycetes are potential sources of novel pharmaceutically active metabolites, significantly advancing natural product research. In the present investigation, secondary metabolites from two endophytic actinomycetes, Streptomyces parvulus GloL3, and Streptomyces lienomycini SK5, isolated from medicinal plant taxa, Globba marantina, and Selaginella kraussiana, exhibited broad-spectrum bioactivity. Ethyl Acetate (EA) extract of SK5 showed antimicrobial activity against nine human pathogens, including Methicillin-resistant Staphylococcus aureus (MRSA), Candida tropicalis, and C.
View Article and Find Full Text PDFEvidence that staphylococcal superantigens promote within-patient bacterial persistence following post-operative surgical site infection.
Infect Immun
January 2025
Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada.
is a predominant cause of post-operative surgical site infections and persistent bacteremia. Here, we describe a patient who experienced three episodes of infection over a period of 4 months following a total knee arthroplasty. The initial bloodstream isolate (SAB-0429) was a clonal complex 5 (CC5) and methicillin-resistant (MRSA), whereas two subsequent isolates (SAB-0485 and SAB-0495) were CC5 isolates but methicillin-sensitive .
View Article and Find Full Text PDF<b>Background and Objective:</b> Peatlands are unique ecosystems rich in microbial diversity, including bacteria with potential antibiotic activity. This study focuses on the isolation and characterization of bacteria from Indonesian peat soil, particularly their potential to produce antibiotics against multidrug-resistant (MDR) pathogens, including Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA). <b>Materials and Methods:</b> Bacterial isolates were rejuvenated on nutrient agar and subjected to antimicrobial activity testing using the Bauer & Kirby diffusion method against MRSA.
View Article and Find Full Text PDFStudying Cationic Liposomes for Quick, Simple, and Effective Nucleic Acid Preconcentration and Isolation.
Anal Chem
January 2025
Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitaetsstr. 31, Regensburg 93053, Germany.
To ensure high quality of food and water, the identification of traces of pathogens is mandatory. Rapid nucleic acid-based tests shorten traditional detection times while maintaining low detection limits. Challenging is the loss of nucleic acids during necessary purification processes, since elution off solid surfaces is not efficient.
View Article and Find Full Text PDF[Effects of Chlorhexidine Gluconate Bathing Applied to Cancer Patients on MRSA and VRE Colonization: A Cross-Over Design Study].
Mikrobiyol Bul
January 2025
Hacettepe Üniversitesi Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Ankara.
Kanıtlar, klorheksidin glukonat (CHG) solüsyonuyla gerçekleştirilen banyonun, sağlık hizmeti ilişkili enfeksiyonlar (SHİE)'a neden olan mikroorganizmaların kolonizasyonunu azalttığını göstermektedir. Bu çalışmada yoğun bakım ünitesi (YBÜ)'nde yatan kanser hastalarında CHG banyosunun MRSA ve VRE kolonizasyonu üzerindeki etkilerinin değerlendirilmesi amaçlanmıştır. Bu çapraz tasarımlı çalışmada, kanser hastalarında standart su + sabun banyosuyla %2'lik CHG banyosu karşılaştırılmıştır.
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