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Enhancing cellulose and hemicellulose degradation in wheat straw composting by inoculation with : key factors and microbial community dynamics.
Environ Technol
January 2025
Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, People's Republic of China.
Actinobacteria are widely used in aerobic composting of straw waste because of their good degradation effect on lignocellulose. However, there are few studies on the degradation effect of on straw. In this study, six laboratory-scale treatments were conducted: corn straw composting with inoculation (CSI), rice straw composting with inoculation (RSI), and wheat straw composting with inoculation (WSI).
View Article and Find Full Text PDFExpression patterns of folate metabolism-related enzymes in the bovine oviduct: estrous cycle-dependent modulation and responsiveness to folic acid.
Theriogenology
December 2024
Laboratorio de Biología Molecular, Facultad de Ciencias Agrarias y Veterinarias, Universidad Católica de Salta (UCASAL), Campus Castañares, A4400EDD, Salta, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address:
Folate metabolism is required for important biochemical processes that regulate cell functioning, but its role in female reproductive physiology in cattle during peri- and post-conceptional periods has not been thoroughly explored. Previous studies have shown the presence of folate in bovine oviductal fluid, as well as finely regulated gene expression of folate receptors and transporters in bovine oviduct epithelial cells (BOECs). Additionally, extracellular folic acid (FA) affects the transcriptional levels of genes important for the functioning of BOECs.
View Article and Find Full Text PDFInteractions between Zoliflodacin and Gyrase and Topoisomerase IV: Enzymological Basis for Cellular Targeting.
ACS Infect Dis
August 2024
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, United States.
Article Synopsis
- * Spiropyrimidinetriones (SPTs), like zoliflodacin, have been developed to fight this resistance and show effectiveness against uncomplicated gonorrhea during human trials.
- * Zoliflodacin is more potent against gyrase than topoisomerase IV, producing more DNA cleavage, and the new SPT H3D-005722 shows promise for achieving balanced targeting of both enzymes.
Biochemical properties and application of a multi-domain β-1,3-1,4-glucanase from Fibrobacter sp.
Int J Biol Macromol
July 2024
School of Life Sciences, Shanghai University, Shanghai 200444, China. Electronic address:
A novel glycoside hydrolase (GH) family 16 multi-domain β-1,3-1,4-glucanase (FsGlc16A) from Fibrobacter sp. UWP2 was identified, heterogeneously expressed, and its enzymatic properties, protein structure and application potential were characterized. Enzymological characterization showed that FsGlc16A performed the optimal catalytic activity at pH 4.
View Article and Find Full Text PDFA novel type IIb L-asparaginase from Latilactobacillus sakei LK-145: characterization and application.
Arch Microbiol
May 2024
Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka-fu, 564-8680, Japan.
We succeeded in homogeneously expressing and purifying L-asparaginase from Latilactobacillus sakei LK-145 (Ls-Asn1) and its mutated enzymes C196S, C264S, C290S, C196S/C264S, C196S/C290S, C264S/C290S, and C196S/C264S/C290S-Ls-Asn1. Enzymological studies using purified enzymes revealed that all cysteine residues of Ls-Asn1 were found to affect the catalytic activity of Ls-Asn1 to varying degrees. The mutation of Cys196 did not affect the specific activity, but the mutation of Cys264, even a single mutation, significantly decreased the specific activity.
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