AI Article Synopsis
- The study investigated the mutagenic effects of ozonated components of humic substances, specifically vanillin, syringaldehyde, vanillic acid, and di-n-butylphtalate, using the Salmonella typhimurium TA 98 and 100 strains.
- Strong mutagenic activity was observed in all components except di-n-butylphtalate when tested with strain TA 100, with or without the S9 mix.
- The strong mutagens resulting from ozonation were primarily water-soluble, had molecular weights greater than 300, and were extracted less effectively with organic solvents like benzene and ethyl acetate.
Article Abstract
Components of humic substances, such as vanillin, syringaldehyde, vanillic acid and di-n-butylphtalate, were ozonated and subjected to the mutagenicity assay using Salmonella typhimurium TA 98 and 100 with and without S9 mix. The strong mutagenic activity was found on all components except di-n-butylphtalate by strain TA 100 with and without S9 mix. Substances with strong mutagenic activity in ozonated vanillin were water-soluble and were slightly extracted with benzene, dichloromethane and ethyl acetate. Following gel chromatography on Sephadex G-10, the strong mutagens generated by ozonation were found with molecular weights greater than 300.
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| http://dx.doi.org/10.1016/0048-9697(92)90361-u | DOI Listing |
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