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[A simplified method of rapid hemagglutination test for determining the levels of tetanus antibodies].
Med Dosw Mikrobiol
March 1992
Zakład Mikrobiologii Wojskowego Instytutu Higieny i Epidemiologii im. Gen. K. Kaczkowskiego w Warszawie.
A new, simplified methodology of preparation of lyophilized chrome-coated turkey blood cells (formalin-treated ) for rapid identification of tetanus antibodies in indirect hemagglutination test is described. Blood cells diagnostic preparations obtained in this way were easier and three times faster to prepare than tanned cell preparations . They maintained unchanged capability for specific agglutination during at least one year of storage when kept at 4 degrees C.
View Article and Find Full Text PDFComparison of tanned cell hemagglutination and counter immunoelectrophoresis test in the detection of antibodies to extractable nuclear antigens (ENA) and to DNA in the systemic lupus erythematosus.
Ann Biol Clin (Paris)
August 1989
Cátedra de Química Clínica II, Universidad Nacional de Córdoba, Argentina.
A comparative study of tanned cell hemagglutination (TCH) and counterimmunoelectrophoresis (CIE), two easy and reliable methodes for the routine detection of antibodies against nuclear antigens was performed. Antibodies against ENA, RNA-ase sensitive ENA and DNA antigens were searched in patients affected by systemic lupus erythematosus (SLE). Analysis of the results obtained for a particular antibody using TCH and CIE techniques suggests that both methods should be used for the detection of antibodies to nuclear antigens since in several cases antibodies were detected by one method and not for the other.
View Article and Find Full Text PDFThermostable ethanol-insoluble antigens of human heart muscle.
Int Arch Allergy Appl Immunol
June 1987
Organ-specific reactions were observed between thermostable ethanol-insoluble (BE) fraction of human heart muscle and its corresponding rabbit antiserum by means of tanned cell hemagglutination test, enzyme immunoassay, and gel precipitation test. Two heart-specific components could be identified using gel precipitation and immunoblotting procedures. Antibodies to human heart muscle BE combined also with heart BE preparations from mammals other than man; these interspecies cross-reactions included also rabbit heart BE pointing to the 'auto' nature of some of the antibodies under investigation.
View Article and Find Full Text PDFA protocol for large-scale screening of blood donors for IgA deficiency using a tanned cell passive haemagglutination-inhibition (PHI) technique and enzyme-linked immunosorbent assay (ELISA) is described. Evidence presented suggests that native serum from a case of IgA paraproteinaemia, suitably diluted and heated to 56 degrees C for 60 min, can act as a satisfactory and simple alternative to purified IgA preparations in the PHI test. When PHI and ELISA techniques were used in combination to screen 41,851 random donors, 58 were found to contain less than 200 ng/ml of IgA and were classified as totally IgA-deficient (1:721).
View Article and Find Full Text PDFIsolation of rat male accessory glands autoantigens by affinity chromatography.
Am J Reprod Immunol (1980)
September 1983
The autoantigens of rat male accessory glands were isolated by a short procedure which involved 1) immunization of rats with chemically modified rat male accessory glands' saline extract; 2) purification of immunoglobulin G (IgG) from the autoantisera by chromatography on DEAE-Sephadex A-50;3) coupling of rat IgG anti-rat male accessory glands with 4-B activated Sepharose; 4) addition of rat male accessory glands' saline extract and removal of autoantigens by glycine-HCl, pH 2.9. The purity of the eluted autoantigens was determined by polyacrylamide disc gel electrophoresis (PAGE).
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