Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Genetic engineering of Nannochloropsis oceanica to produce canthaxanthin and ketocarotenoids.
Microb Cell Fact
November 2024
Dipartimento di Biotecnologie, Università Degli Studi di Verona, Strada le Grazie 15, 37134, Verona, Italy.
Article Synopsis
- * Researchers engineered N. oceanica using a specific strain (LP-tdTomato) to enhance ketocarotenoid production by introducing a key enzyme (CrBKT) from another alga, resulting in significant increases in carotenoid levels and a noticeable orange/red coloration in the cultures.
- * Optimal production of canthaxanthin and ketocarotenoids was achieved at lower light intensity (150 µmol m s),
Different relative scalings between transient forces and thermal fluctuations tune regimes of chromatin organization.
ArXiv
June 2024
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27510.
Within the nucleus, structural maintenance of chromosome protein complexes, namely condensin and cohesin, create an architecture to facilitate the organization and proper function of the genome. Condensin, in addition to performing loop extrusion, creates localized clusters of chromatin in the nucleolus through transient crosslinks. Large-scale simulations revealed three different dynamic behaviors as a function of timescale: slow crosslinking leads to no clusters, fast crosslinking produces rigid slowly changing clusters, while intermediate timescales produce flexible clusters that mediate gene interaction.
View Article and Find Full Text PDFThe cytidine deaminase APOBEC3A regulates nucleolar function to promote cell growth and ribosome biogenesis.
PLoS Biol
July 2024
Department of Molecular Biophysics & Biochemistry, Yale University School of Medicine, New Haven, Connecticut, United States of America.
Cancer initiates as a consequence of genomic mutations and its subsequent progression relies in part on increased production of ribosomes to maintain high levels of protein synthesis for unchecked cell growth. Recently, cytidine deaminases have been uncovered as sources of mutagenesis in cancer. In an attempt to form a connection between these 2 cancer driving processes, we interrogated the cytidine deaminase family of proteins for potential roles in human ribosome biogenesis.
View Article and Find Full Text PDFHigh plasticity of ribosomal DNA organization in budding yeast.
Cell Rep
February 2024
CAS Key Laboratory of Quantitative Engineering Biology, Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China; College of Life Sciences and Oceanography, Shenzhen University, 1066 Xueyuan Road, Shenzhen 518055, China. Electronic address:
In eukaryotic genomes, rDNA generally resides as a highly repetitive and dynamic structure, making it difficult to study. Here, a synthetic rDNA array on chromosome III in budding yeast was constructed to serve as the sole source of rRNA. Utilizing the loxPsym site within each rDNA repeat and the Cre recombinase, we were able to reduce the copy number to as few as eight copies.
View Article and Find Full Text PDFDynamic mapping of proteome trafficking within and between living cells by TransitID.
Cell
July 2023
Departments of Biology, Genetics, and Chemistry, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub-San Francisco, San Francisco, CA 94158, USA. Electronic address:
The ability to map trafficking for thousands of endogenous proteins at once in living cells would reveal biology currently invisible to both microscopy and mass spectrometry. Here, we report TransitID, a method for unbiased mapping of endogenous proteome trafficking with nanometer spatial resolution in living cells. Two proximity labeling (PL) enzymes, TurboID and APEX, are targeted to source and destination compartments, and PL with each enzyme is performed in tandem via sequential addition of their small-molecule substrates.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!