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Applying a Fluorescence Polarization Assay for Detection of Brucellosis in Animals Using the Fluorescently Labeled Synthetic Oligosaccharides as Biosensing Tracer.
Biosensors (Basel)
August 2024
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
Brucellosis in animals is an infectious disease caused by bacteria of the genus . Known methods for diagnosing brucellosis face some challenges, due to the difficulties in isolating and standardizing the natural brucellosis antigen. In this work, we investigated the possibility of using the fluorescence polarization assay (FPA) with synthetic glycoconjugate biosensing tracers to detect antibodies against as a new methodology for diagnosing brucellosis.
View Article and Find Full Text PDFBacteroidota polysaccharide utilization system for branched dextran exopolysaccharides from lactic acid bacteria.
J Biol Chem
July 2023
Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, Suruga-ku, Shizuoka, Japan; Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Suruga-ku, Shizuoka, Japan; Research Institute of Green Science and Technology, Shizuoka University, Suruga-ku, Shizuoka, Japan. Electronic address:
Dextran is an α-(1→6)-glucan that is synthesized by some lactic acid bacteria, and branched dextran with α-(1→2)-, α-(1→3)-, and α-(1→4)-linkages are often produced. Although many dextranases are known to act on the α-(1→6)-linkage of dextran, few studies have functionally analyzed the proteins involved in degrading branched dextran. The mechanism by which bacteria utilize branched dextran is unknown.
View Article and Find Full Text PDFIdentification and Characterization of Dextran α-1,2-Debranching Enzyme from .
J Appl Glycosci (1999)
March 2023
5 Faculty of Life and Environmental Sciences, University of Yamanashi.
Dextran α-1,2-debranching enzyme (DDE) releases glucose with hydrolyzing α-(1→2)-glucosidic linkages in α-glucans, which are made up of dextran with α-(1→2)-branches and are generated by bacteria. DDE was isolated from (formerly known as sp. M-73) 40 years ago, although the amino acid sequence of the enzyme has not been determined.
View Article and Find Full Text PDFGlycoside hydrolases active on microbial exopolysaccharide α-glucans: structures and function.
Essays Biochem
April 2023
Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
Glucose is the most abundant monosaccharide in nature and is an important energy source for living organisms. Glucose exists primarily as oligomers or polymers and organisms break it down and consume it. Starch is an important plant-derived α-glucan in the human diet.
View Article and Find Full Text PDFStructural characterization and partial properties of dextran produced by RSG7 from pepino.
Front Microbiol
February 2023
School of Life Sciences, Shanxi Normal University, Taiyuan, China.
Exopolysaccharides (EPSs) produced by lactic acid bacteria possess various bioactivities and potential attractions for scientific exploration and commercial development. An EPS-producing bacterial strain, RSG7, was previously isolated from the pepino and identified as . Based on the analyses of high-performance size exclusion chromatography, high-performance ion chromatography, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and methylation, the RSG7 EPS was identified as a dextran with a molecular weight of 5.
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