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Seed bacterization with siderophore-producing bacteria: a strategy to enhance growth and alkaloid content in Catharanthus roseus.

World J Microbiol Biotechnol

January 2025

Department of Biotechnology and Bioengineering, Institute of Advanced Research, Koba Institutional Area, Gandhinagar, Gujarat, 382426, India.

Catharanthus roseus is a medicinal plant widely known for producing monoterpenoid indole alkaloids (MIAs), including therapeutic compounds such as vinblastine and vincristine, which are crucial for cancer treatment. However, the naturally low concentration of these alkaloids in plant tissues poses a significant challenge for large-scale production. This study explores the application of siderophore-producing bacteria for seed bacterization of Catharanthus roseus to enhance the production of MIAs, including vindoline, catharanthine, and vinblastine.

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Ensuring species integrity and successful reproduction is pivotal for the survival of angiosperms. Members of Brassicaceae family employ a "lock and key" mechanism involving stigmatic (sRALFs) and pollen RALFs (pRALFs) binding to FERONIA, a Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) receptor, to establish a prezygotic hybridization barrier. In the absence of compatible pRALFs, sRALFs bind to FERONIA, inducing a lock state for pollen tube penetration.

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Catharanthus roseus leaves have been traditionally described to possess potent antidiabetic activity and some leaf-specific alkaloids, including vindoline, have been studied for their antidiabetic potential. The aim of the present study was to validate the antidiabetic property of the plant with special reference to vindoline. An Ayurveda-based method was used to prepare the Swaras [leaf juice extract (LJE)] of three familial C.

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This study aimed to increase the concentrations of vindoline (VDL) and catharanthine (CAT) in Catharanthus roseus plants cultivated in an indoor farming system using artificial lighting and plasma-activated water (PAW). After a 61-days pre-treatment period under fluorescent lamps, plants were exposed to four treatments: white light (W) from the same fluorescent lamps, red light (R) from LEDs, W with PAW, and R with PAW. These combinations were evaluated at two sampling times: 45 days (T1) and 70 days (T2) after the end of pre-treatment (DAP).

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Background: Sub-Saharan Africa faces one of the highest burdens of venereal diseases (VDs) globally. This review aims to critically evaluate the existing literature on the diverse Indigenous knowledge and medicinal plants utilised for treating VDs in sub-Saharan Africa.

Methods: We used the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) protocol to guide the execution of the review.

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