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investigation of human salivary microbial growth with lysogeny broth for translational research-A pilot study.
J Dent Sci
January 2025
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Background/purpose: Salivary microbiome has become a surrogate indicator of oral disease due to its collective reservoirs and convenience in sampling. However, failed clinical trials often lead to wastes of resources, indicating a need for preclinical models. In this pilot study, we aimed to compare the salivary microbiome by metagenomics analysis before and after lysogeny broth culture for prospective translational studies.
View Article and Find Full Text PDFTesting the Purity of Cultures After Axenicity Treatments.
Cells
January 2025
Department of Functional and Evolutionary Ecology, University of Vienna, Djerassiplatz 1, A-1030 Vienna, Austria.
Contaminations are challenging for monocultures, as they impact the culture conditions and thus influence the growth of the target organism and the overall biomass composition. In phycology, axenic cultures comprising a single living species are commonly strived for both basic research and industrial applications, because contaminants reduce significance for analytic purposes and interfere with the safety and quality of commercial products. We aimed to establish axenic cultures of , known as the food additive "Spirulina".
View Article and Find Full Text PDFPhenotypic and Transcriptomic Changes in Acinetobacter baumannii in Rich and Minimal Growth Mediums.
Can J Microbiol
January 2025
University of Manitoba, Department of Microbiology, Winnipeg, Canada;
Acinetobacter baumannii is an opportunistic pathogen that is often studied in commonly used rich media in laboratories worldwide. Due to the metabolic versatility of A. baumannii, it can be cultured in different growth mediums; however, this can lead to genotypic and phenotypic variations.
View Article and Find Full Text PDFComplete genome sequence of Vibrio diabolicus bacteriophage vB_Vc_SrVc2 and its efficacy as prophylactic phage therapy.
Virology
January 2025
Centro de Investigaciones Biológicas del Noroeste S.C, Av. Instituto Politécnico Nacional 195. Col. Playa Palo de Santa Rita Sur, 23096, La Paz, Baja California Sur, Mexico. Electronic address:
Vibrio diabolicus is widely distributed in the marine environment and is an important pathogen of aquatic organisms such as shrimp, fish, and mollusks. The emergence of multi-drug resistance among these bacteria has resulted in a global public health problem, which requires alternative treatment approaches, such as phage therapy. In the present study, we isolated the phage vB_Vc_SrVc2 from the hepatopancreas of white shrimp showing symptoms of acute hepatopancreatic necrosis disease (AHPND) and evaluated the efficacy of this phage in preventing the mortality associated with V.
View Article and Find Full Text PDFPathogenicity and identification of host adaptation genes of the avian pathogenic O145 in duck.
Front Cell Infect Microbiol
November 2024
Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang, China.
Article Synopsis
- APEC O145 is a significant bacterial pathogen impacting poultry in China, with recent studies indicating its rise as a predominant serogroup.
- A virulent strain called NC22 was analyzed to understand its pathogenic mechanisms through whole-genome sequencing and by observing its behavior in duck liver compared to lab cultures.
- The study found that NC22 infection led to severe symptoms in ducks and identified 87 differentially expressed genes related to metabolism and host adaptation, shedding light on the complex interactions between APEC O145 and its hosts.
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