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Spectral Regularities of Viscoelastic Parameters of Whole Blood Exposed to Periodic Shear Stress.
Bull Exp Biol Med
June 2020
National Research Tomsk State University, Tomsk, Russia.
Theoretic and experimental study of viscoelastic properties of the whole blood exposed to shear stress was carried out with acoustic resonance method based on the measurement of gain-frequency characteristics of resonating needle in an ARP-01M piezoelectric thromboelastograph (Mednord). The study revealed regularities in the changes of viscoelastic parameters of the whole blood within 0-80 kHz frequency range of shear vibrations. In this frequency range, the elastic (storage) modulus G' reflecting blood elasticity increased with frequency and significantly contributed to the complex viscosity coefficient.
View Article and Find Full Text PDFMembrane action of polyhexamethylene guanidine hydrochloride revealed on smooth muscle cells, nerve tissue and rat blood platelets: A biocide driven pore-formation in phospholipid bilayers.
Toxicol In Vitro
October 2019
Department of Muscle Biochemistry, Palladin Institute of Biochemistry, NAS of Ukraine, Leontovich Str., 9, 01030 Kyiv, Ukraine.
A well-known cationic biocide of guanidine polymer family, polyhexamethylene guanidine hydrochloride (PHMG) has been tested against smooth muscle cells isolated from swine myometrium, synaptosomes of rat brain nerve terminals and rat blood platelets for the membrane action. It was established that PHMG blocked the activity of Na,K-ATPase of smooth muscle cells plasma membrane by 82.2 ± 0.
View Article and Find Full Text PDFIndirect viscosimetric method is less accurate than ektacytometry for the measurement of red blood cell deformability.
Clin Hemorheol Microcirc
November 2015
UMR Inserm 665, Pointe-à-Pitre, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe, France Laboratoire ACTES (EA 3596), Département de Physiologie, Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe, France Laboratory of Excellence GR-Ex «The red cell: from genesis to death», PRES Sorbonne Paris Cité, Paris, France.
The aim of this study was to test the accuracy of viscosimetric method to estimate the red blood cell (RBC) deformability properties. Thirty-three subjects were enrolled in this study: 6 healthy subjects (AA), 11 patients with sickle cell-hemoglobin C disease (SC) and 16 patients with sickle cell anemia (SS). Two methods were used to assess RBC deformability: 1) indirect viscosimetric method and 2) ektacytometry.
View Article and Find Full Text PDFWe assessed effect of 6-weeks therapy with atorvastatin on parameters of lipid metabolism and some hemorheological characteristics in 27 patients with arterial hypertension (AH) and dyslipoproteinemia (DLP). In addition to general clinical examination we studied hemorheological profile including hematocrit, blood plasma and whole blood viscosity with the method of rotational viscosimetry at various shear rates, deformability of erythrocytes by filtration method, and cytoarchitectonics of erythrocytes. At the background of pronounced lipid lowering effect of atorvastatin we noted obvious improvement of microrheological properties of erythrocytes appearing as significant decrease of rigidity index of erythrocytes and of their aggregation.
View Article and Find Full Text PDFDeformability is an important property of red blood cells and ensures the efficiency of their transport in the microcirculatory vascular system, as well as tissue oxygenation. A study of erythrocyte deformability uses different methods: filtration through about 5.0-microm pores, laser difractometry techniques and a focus of attention has recently been on a procedure for measuring the deformability of erythrocytes in a flow microchamber.
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