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J Mech Behav Biomed Mater
January 2014
Materials Science Program, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address:
Polymethyl methacrylate bone cement is the most common and successful method used to anchor orthopedic implants to bone, as evidenced by data from long-term national joint registries. Despite these successes, mechanical failure of the cement mantle can result in premature failure of an implant which has lead to the development of a variety of techniques aimed at enhancing the mechanical properties of the cement, such as the addition of particulate or fiber reinforcements. This technique however has not transitioned into clinical practice, likely due to problems relating to interfacial particle/matrix adhesion and high cement stiffness.
View Article and Find Full Text PDFJ Forensic Dent Sci
January 2013
Departments of Oral Medicine, Diagnosis, and Radiology, Dayananda Sagar College of Dental Sciences and Hospital, Shavige Malleswara Hills, Bangalore, Karnataka, India.
Background: Teeth have always been known as stable tissue that can be preserved both physically and chemically for long periods of time. Blood group substances have been known to be present in both the hard and soft tissues of the teeth.
Objectives: This study aimed at detection of ABO blood group substances from soft and hard tissues of teeth and also to evaluate the reliability of teeth stored for a relatively long period as a source of blood group substances by absorption-elution technique with some modifications.
The proposed method for the specific detection of Rhesus D antigen in 6 month old bloodstains is based on the use of modified immunoglobulins (anti-rhesus D peptides) in the absorption-elution reaction (AER). These peptides were prepared by an original method using polyclonal anti-rhesus D antibodies and standard erythrocytes preliminarily treated with a highly active protease. Specific identification of D antigen of the Rhesus system obviates the problem of poor AER specificity and thereby significantly improves the quality of forensic biological expertise.
View Article and Find Full Text PDFVox Sang
August 2010
Organ and Tissue Transplantation Immunology, Fondazione IRCCS, Ospedale Maggiore Policlinico, Mangiagalli, Regina Elena, via Francesco Sforza 35, Milan, Italy.
Background: The antigens of the Colton blood group system, Co(a) and Co(b), are encoded by a single gene that produces the aquaporin-1 (AQP1) protein, a water channel-forming protein, and are characterized by a single nucleotide polymorphism (SNP). A healthy Caucasoid blood donor originally typed as Co(a-b-) with commercial anti-Co(b) typed Co(a-b+) when retested with another anti-Co(b). Retyped with two different molecular biology methods, the sample came out Co(a)/Co(b).
View Article and Find Full Text PDFZhongguo Shi Yan Xue Ye Xue Za Zhi
August 2007
Department of Blood Transfusion, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
The study was to investigate the characteristics of Rh blood group of Uygur nationality in Xinjiang. 1 230 blood samples of Uygur nationality were studied by Rh serological typing, modified antiglobulin test, chloroform/trichloroethylene absorption elution test, upstream, downstream and hybrid Rhesus boxes, 10 exons of D gene, RHD(psi) pseudogene. The results showed that the frequency of RHD negative was 5.
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